Detailed information of TA system TA030059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3064230..3064909
• Replicon: chromosome
• Accession: NC_022350
• Organism: Mycobacterium tuberculosis str. Haarlem

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: TBHG_RS14450
• Protein ID: WP_003414059.1
• Coordinates: 3064230..3064646 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: TBHG_RS14455
• Protein ID: WP_003414061.1
• Coordinates: 3064643..3064909 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBHG_RS14430	3060282..3061184	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
TBHG_RS14435	3061253..3062005	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
TBHG_RS14440	3062249..3062524	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
TBHG_RS14445	3062521..3064143	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
TBHG_RS14450	3064230..3064646	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
TBHG_RS14455	3064643..3064909	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBHG_RS14460	3064935..3065330	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
TBHG_RS14465	3065327..3065596	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
TBHG_RS14470	3065606..3066700	-	1095	WP_003900565.1	restriction endonuclease subunit S	-
TBHG_RS14475	3066697..3067116	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
TBHG_RS14480	3067190..3067669	-	480	WP_003414073.1	dihydrofolate reductase	-
TBHG_RS14485	3067740..3068540	-	801	WP_003911953.1	thymidylate synthase	-
TBHG_RS14490	3068696..3069433	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T30059 WP_003414059.1 NC_022350:c3064646-3064230 [Mycobacterium tuberculosis str. Haarlem]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T30059 NC_022350:c3064646-3064230 [Mycobacterium tuberculosis str. Haarlem]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT30059 WP_003414061.1 NC_022350:c3064909-3064643 [Mycobacterium tuberculosis str. Haarlem]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT30059 NC_022350:c3064909-3064643 [Mycobacterium tuberculosis str. Haarlem]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64