Detailed information of TA system TA030041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194275..2194978
• Replicon: chromosome
• Accession: NC_022350
• Organism: Mycobacterium tuberculosis str. Haarlem

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: TBHG_RS10120
• Protein ID: WP_003409778.1
• Coordinates: 2194275..2194604 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: TBHG_RS21250
• Protein ID: WP_003409780.1
• Coordinates: 2194601..2194978 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBHG_RS10100	2190658..2191728	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
TBHG_RS10105	2191725..2192240	+	516	WP_003409718.1	flavin reductase family protein	-
TBHG_RS10110	2192237..2193298	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
TBHG_RS10115	2193295..2194065	+	771	WP_003409775.1	SDR family oxidoreductase	-
TBHG_RS10120	2194275..2194604	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TBHG_RS21250	2194601..2194978	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
TBHG_RS10130	2194975..2195565	-	591	WP_003409784.1	SEC-C domain-containing protein	-
TBHG_RS10135	2195620..2196984	+	1365	WP_003903691.1	HNH endonuclease	-
TBHG_RS10140	2197139..2197591	-	453	WP_003899095.1	lipoprotein	-
TBHG_RS10145	2197655..2198056	+	402	WP_003409869.1	hypothetical protein	-
TBHG_RS10150	2198049..2198231	-	183	WP_003409870.1	hypothetical protein	-
TBHG_RS10155	2198345..2198695	-	351	WP_003899096.1	hypothetical protein	-
TBHG_RS10160	2198706..2199608	-	903	WP_003899097.1	hypothetical protein	-
TBHG_RS10165	2199629..2199820	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T30041 WP_003409778.1 NC_022350:c2194604-2194275 [Mycobacterium tuberculosis str. Haarlem]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T30041 NC_022350:c2194604-2194275 [Mycobacterium tuberculosis str. Haarlem]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT30041 WP_003409780.1 NC_022350:c2194978-2194601 [Mycobacterium tuberculosis str. Haarlem]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT30041 NC_022350:c2194978-2194601 [Mycobacterium tuberculosis str. Haarlem]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8