Detailed information of TA system TA030038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1767051..1767664
• Replicon: chromosome
• Accession: NC_022350
• Organism: Mycobacterium tuberculosis str. Haarlem

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: TBHG_RS08270
• Protein ID: WP_003407786.1
• Coordinates: 1767260..1767664 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: TBHG_RS08265
• Protein ID: WP_009935474.1
• Coordinates: 1767051..1767254 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBHG_RS08235	1762456..1762836	+	381	WP_003407768.1	fumarate reductase subunit C	-
TBHG_RS08240	1762833..1763210	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
TBHG_RS08245	1763278..1763886	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
TBHG_RS08250	1764070..1765218	+	1149	Protein_1615	MMPL family transporter	-
TBHG_RS08255	1765228..1765674	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
TBHG_RS08260	1765709..1766998	+	1290	WP_003407781.1	threonine ammonia-lyase	-
TBHG_RS08265	1767051..1767254	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBHG_RS08270	1767260..1767664	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
TBHG_RS08275	1767681..1769423	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
TBHG_RS08280	1769416..1771713	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T30038 WP_003407786.1 NC_022350:1767260-1767664 [Mycobacterium tuberculosis str. Haarlem]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T30038 NC_022350:1767260-1767664 [Mycobacterium tuberculosis str. Haarlem]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT30038 WP_009935474.1 NC_022350:1767051-1767254 [Mycobacterium tuberculosis str. Haarlem]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT30038 NC_022350:1767051-1767254 [Mycobacterium tuberculosis str. Haarlem]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46