Detailed information of TA system TA030033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1389452..1390140
• Replicon: chromosome
• Accession: NC_022350
• Organism: Mycobacterium tuberculosis str. Haarlem

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: TBHG_RS06580
• Protein ID: WP_003406304.1
• Coordinates: 1389709..1390140 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: TBHG_RS06575
• Protein ID: WP_003406302.1
• Coordinates: 1389452..1389712 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBHG_RS06555	1385029..1385853	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
TBHG_RS06560	1385858..1387039	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
TBHG_RS06565	1387116..1388216	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
TBHG_RS06570	1388387..1389376	+	990	WP_003406301.1	malate dehydrogenase	-
TBHG_RS06575	1389452..1389712	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
TBHG_RS06580	1389709..1390140	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBHG_RS06585	1390163..1391851	-	1689	WP_003910308.1	PE family protein	-
TBHG_RS06590	1392031..1392891	+	861	WP_003900301.1	hypothetical protein	-
TBHG_RS06595	1392972..1393802	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
TBHG_RS06600	1393859..1394152	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
TBHG_RS06605	1394149..1394418	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T30033 WP_003406304.1 NC_022350:1389709-1390140 [Mycobacterium tuberculosis str. Haarlem]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T30033 NC_022350:1389709-1390140 [Mycobacterium tuberculosis str. Haarlem]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT30033 WP_003406302.1 NC_022350:1389452-1389712 [Mycobacterium tuberculosis str. Haarlem]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT30033 NC_022350:1389452-1389712 [Mycobacterium tuberculosis str. Haarlem]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6