Detailed information of TA system TA030028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1018260..1018879
• Replicon: chromosome
• Accession: NC_022350
• Organism: Mycobacterium tuberculosis str. Haarlem

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: TBHG_RS04785
• Protein ID: WP_003404726.1
• Coordinates: 1018445..1018879 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: TBHG_RS04780
• Protein ID: WP_003898641.1
• Coordinates: 1018260..1018439 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBHG_RS04770	1013733..1015313	+	1581	WP_003909313.1	serine hydrolase	-
TBHG_RS04775	1015310..1017703	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
TBHG_RS22305	1017976..1018134	+	159	WP_021036370.1	hypothetical protein	-
TBHG_RS04780	1018260..1018439	+	180	WP_003898641.1	antitoxin	Antitoxin
TBHG_RS04785	1018445..1018879	+	435	WP_003404726.1	SRPBCC family protein	Toxin
TBHG_RS04790	1018977..1019750	+	774	WP_003404735.1	VOC family protein	-
TBHG_RS04795	1019815..1020264	+	450	WP_003404738.1	hypothetical protein	-
TBHG_RS04800	1020399..1020629	-	231	WP_003898642.1	hypothetical protein	-
TBHG_RS04805	1020796..1022304	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
TBHG_RS04810	1022306..1023544	-	1239	WP_003900233.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T30028 WP_003404726.1 NC_022350:1018445-1018879 [Mycobacterium tuberculosis str. Haarlem]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T30028 NC_022350:1018445-1018879 [Mycobacterium tuberculosis str. Haarlem]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT30028 WP_003898641.1 NC_022350:1018260-1018439 [Mycobacterium tuberculosis str. Haarlem]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT30028 NC_022350:1018260-1018439 [Mycobacterium tuberculosis str. Haarlem]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5