Detailed information of TA system TA030019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 707705..708348
• Replicon: chromosome
• Accession: NC_022350
• Organism: Mycobacterium tuberculosis str. Haarlem

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: TBHG_RS03180
• Protein ID: WP_003403187.1
• Coordinates: 707947..708348 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: TBHG_RS03175
• Protein ID: WP_003403184.1
• Coordinates: 707705..707950 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBHG_RS03140	702985..703515	-	531	WP_003917320.1	two component system sensor kinase HK2	-
TBHG_RS03145	703499..704260	-	762	WP_003403148.1	two-component system response regulator TcrA	-
TBHG_RS03150	704317..704628	+	312	WP_003403164.1	hypothetical protein	-
TBHG_RS03155	704700..705650	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
TBHG_RS03160	705891..706475	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
TBHG_RS03165	706477..707187	+	711	Protein_629	transposase	-
TBHG_RS03170	707190..707660	+	471	WP_003898523.1	hypothetical protein	-
TBHG_RS03175	707705..707950	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBHG_RS03180	707947..708348	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBHG_RS21975	708736..708903	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
TBHG_RS21980	708936..709133	-	198	WP_003403191.1	hypothetical protein	-
TBHG_RS03195	709213..710370	-	1158	WP_003900186.1	hypothetical protein	-
TBHG_RS03200	710422..710643	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
TBHG_RS03205	710785..711390	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T30019 WP_003403187.1 NC_022350:707947-708348 [Mycobacterium tuberculosis str. Haarlem]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T30019 NC_022350:707947-708348 [Mycobacterium tuberculosis str. Haarlem]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT30019 WP_003403184.1 NC_022350:707705-707950 [Mycobacterium tuberculosis str. Haarlem]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT30019 NC_022350:707705-707950 [Mycobacterium tuberculosis str. Haarlem]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91