Detailed information of TA system TA030018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 701615..702261
• Replicon: chromosome
• Accession: NC_022350
• Organism: Mycobacterium tuberculosis str. Haarlem

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: TBHG_RS03125
• Protein ID: WP_003403137.1
• Coordinates: 701615..702028 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: TBHG_RS03130
• Protein ID: WP_003403139.1
• Coordinates: 702025..702261 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBHG_RS03105	697698..699248	+	1551	WP_003403119.1	MCE family protein	-
TBHG_RS03110	699300..699692	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
TBHG_RS03115	699689..699946	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
TBHG_RS03120	700129..701364	-	1236	WP_003403128.1	ATP-binding protein	-
TBHG_RS03125	701615..702028	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBHG_RS03130	702025..702261	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TBHG_RS03135	702365..702871	-	507	WP_003900973.1	two component system sensor kinase HK1	-
TBHG_RS03140	702985..703515	-	531	WP_003917320.1	two component system sensor kinase HK2	-
TBHG_RS03145	703499..704260	-	762	WP_003403148.1	two-component system response regulator TcrA	-
TBHG_RS03150	704317..704628	+	312	WP_003403164.1	hypothetical protein	-
TBHG_RS03155	704700..705650	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
TBHG_RS03160	705891..706475	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
TBHG_RS03165	706477..707187	+	711	Protein_629	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T30018 WP_003403137.1 NC_022350:c702028-701615 [Mycobacterium tuberculosis str. Haarlem]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T30018 NC_022350:c702028-701615 [Mycobacterium tuberculosis str. Haarlem]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT30018 WP_003403139.1 NC_022350:c702261-702025 [Mycobacterium tuberculosis str. Haarlem]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT30018 NC_022350:c702261-702025 [Mycobacterium tuberculosis str. Haarlem]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89