Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 699300..699946 Replicon chromosome
Accession NC_022350
Organism Mycobacterium tuberculosis str. Haarlem

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag TBHG_RS03110 Protein ID WP_003403122.1
Coordinates 699300..699692 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag TBHG_RS03115 Protein ID WP_003403125.1
Coordinates 699689..699946 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBHG_RS03095 694961..696397 + 1437 WP_003900182.1 virulence factor Mce family protein -
TBHG_RS03100 696551..697693 + 1143 WP_003911253.1 virulence factor Mce family protein -
TBHG_RS03105 697698..699248 + 1551 WP_003403119.1 MCE family protein -
TBHG_RS03110 699300..699692 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
TBHG_RS03115 699689..699946 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
TBHG_RS03120 700129..701364 - 1236 WP_003403128.1 ATP-binding protein -
TBHG_RS03125 701615..702028 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
TBHG_RS03130 702025..702261 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
TBHG_RS03135 702365..702871 - 507 WP_003900973.1 two component system sensor kinase HK1 -
TBHG_RS03140 702985..703515 - 531 WP_003917320.1 two component system sensor kinase HK2 -
TBHG_RS03145 703499..704260 - 762 WP_003403148.1 two-component system response regulator TcrA -
TBHG_RS03150 704317..704628 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T30017 WP_003403122.1 NC_022350:c699692-699300 [Mycobacterium tuberculosis str. Haarlem]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T30017 NC_022350:c699692-699300 [Mycobacterium tuberculosis str. Haarlem]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT30017 WP_003403125.1 NC_022350:c699946-699689 [Mycobacterium tuberculosis str. Haarlem]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT30017 NC_022350:c699946-699689 [Mycobacterium tuberculosis str. Haarlem]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References