Detailed information of TA system TA030016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 682170..682789
• Replicon: chromosome
• Accession: NC_022350
• Organism: Mycobacterium tuberculosis str. Haarlem

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O53779
• Locus tag: TBHG_RS03045
• Protein ID: WP_003403047.1
• Coordinates: 682382..682789 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O53778
• Locus tag: TBHG_RS03040
• Protein ID: WP_003403039.1
• Coordinates: 682170..682385 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBHG_RS03030	680693..681451	+	759	WP_003898511.1	Mut7-C ubiquitin/RNAse domain-containing protein	-
TBHG_RS03035	681580..682071	-	492	WP_003403017.1	hypothetical protein	-
TBHG_RS03040	682170..682385	+	216	WP_003403039.1	type II toxin-antitoxin system antitoxin VapB26	Antitoxin
TBHG_RS03045	682382..682789	+	408	WP_003403047.1	type II toxin-antitoxin system toxin ribonuclease C26	Toxin
TBHG_RS03050	682849..683535	-	687	WP_003403052.1	LpqN/LpqT family lipoprotein	-
TBHG_RS03055	683689..686322	+	2634	WP_003403055.1	GH92 family glycosyl hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-39)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14471.43 Da
• Isoelectric Point: 4.4493

>T30016 WP_003403047.1 NC_022350:682382-682789 [Mycobacterium tuberculosis str. Haarlem]
VIIDTSALLAYFDAAEPDHAAVSECIDSSADALVVSPYVVAELDYLVATRVGVDAELAVLRELAGGAWELANCGAAEIEQ
AARIVTKYQDQRIGIADAANVVLADRYRTRTILTLDRRHFSALRPIGGGRFTVIP

Nucleotide

• Length: 408 bp

>T30016 NC_022350:682382-682789 [Mycobacterium tuberculosis str. Haarlem]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7572.66 Da
• Isoelectric Point: 10.5454

>AT30016 WP_003403039.1 NC_022350:682170-682385 [Mycobacterium tuberculosis str. Haarlem]
VDKTTVYLPDELKAAVKRAARQRGVSEAQVIRESIRAAVGGAKPPPRGGLYAGSEPIARRVDELLAGFGER

Nucleotide

• Length: 216 bp

>AT30016 NC_022350:682170-682385 [Mycobacterium tuberculosis str. Haarlem]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Structures

Toxin

• PDB: 5X3T

Antitoxin

• AlphaFold DB: A0A7U4BSA2