Detailed information of TA system TA030013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 366987..367630
• Replicon: chromosome
• Accession: NC_022350
• Organism: Mycobacterium tuberculosis str. Haarlem

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB8
• Locus tag: TBHG_RS01590
• Protein ID: WP_003401566.1
• Coordinates: 367205..367630 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: TBHG_RS01585
• Protein ID: WP_003401563.1
• Coordinates: 366987..367208 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBHG_RS01560	362106..362909	-	804	WP_003401540.1	hypothetical protein	-
TBHG_RS01565	362919..364316	-	1398	WP_003401544.1	sulfatase	-
TBHG_RS01570	364495..366270	+	1776	WP_010886074.1	PE family protein	-
TBHG_RS01575	366413..366640	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
TBHG_RS01580	366637..366939	+	303	WP_003401560.1	toxin-antitoxin system toxin	-
TBHG_RS01585	366987..367208	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
TBHG_RS01590	367205..367630	+	426	WP_003401566.1	PIN domain nuclease	Toxin
TBHG_RS01595	367766..368398	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
TBHG_RS01600	368395..369303	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-125)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15713.90 Da
• Isoelectric Point: 5.6002

>T30013 WP_003401566.1 NC_022350:367205-367630 [Mycobacterium tuberculosis str. Haarlem]
VTDQRWLIDKSALVRLTDSPDMEIWSNRIERGLVHITGVTRLEVGFSAECGEIARREFREPPLSAMPVEYLTPRIEDRAL
EVQTLLADRGHHRGPSIPDLLIAATAELSGLTVLHVDKDFDAIAALTGQKTERLTHRPPSA

Nucleotide

• Length: 426 bp

>T30013 NC_022350:367205-367630 [Mycobacterium tuberculosis str. Haarlem]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT30013 WP_003401563.1 NC_022350:366987-367208 [Mycobacterium tuberculosis str. Haarlem]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT30013 NC_022350:366987-367208 [Mycobacterium tuberculosis str. Haarlem]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Toxin

• PDB: 3H87

Antitoxin

• AlphaFold DB: A0A829CG48