30004

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 149388..150735 Replicon chromosome
Accession NC_022270
Organism Vibrio campbellii ATCC BAA-1116

Toxin (Protein)


Gene name sanaT Uniprot ID A7N7V0
Locus tag M892_RS18020 Protein ID WP_012130176.1
Coordinates 149794..150735 (+) Length 314 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID A0A1B1NML2
Locus tag M892_RS18015 Protein ID WP_021018080.1
Coordinates 149388..149792 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M892_RS17980 144460..145440 + 981 WP_021018078.1 site-specific integrase -
M892_RS17985 145471..146391 + 921 WP_012130183.1 IS5 family transposase -
M892_RS17990 146388..147032 - 645 WP_012130182.1 DUF4150 domain-containing protein -
M892_RS17995 147050..147502 - 453 WP_021018079.1 hypothetical protein -
M892_RS18005 148084..148326 + 243 WP_012130179.1 hypothetical protein -
M892_RS18010 148606..149199 + 594 WP_012130178.1 tyrosine-type recombinase/integrase -
M892_RS18015 149388..149792 + 405 WP_021018080.1 hypothetical protein Antitoxin
M892_RS18020 149794..150735 + 942 WP_012130176.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
M892_RS18025 150755..152302 + 1548 WP_021018081.1 type I restriction-modification system subunit M -
M892_RS30005 152397..152564 + 168 Protein_147 restriction endonuclease subunit S -
M892_RS18035 152630..153391 + 762 WP_012130173.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 133155..153391 20236


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(32-300)

Antitoxin

Protein of unknown function DUF6088

(7-130)


Sequences

Toxin        


Download         Length: 314 a.a.        Molecular weight: 35743.17 Da        Isoelectric Point: 5.2980

>T30004 WP_012130176.1 NC_022270:149794-150735 [Vibrio campbellii ATCC BAA-1116]
MNTLKQQFLEVSDALELGNPAIIEKDYWVVTLLAELAKVSPEHHQMVFSGGTALAKSNVKILRMSEDVDIKLIPNPAFDE
LSRAKKKTARKACVNEIEQALTQSGLFNVETRSVRDEYRYVEFELKYPQQFSQAPCLRPIIKLELIETIPLLDVESRSIQ
SLVAELYQQPQEVSAFDCVSIHATLVEKIISMLRRTMSVKRNTERSDDVALVRHIYDVHCITKLAMPELNEIDGLFKAVL
AEDIARFGNQHAEFVANPKQELQLGLKELEENAIFRQRFQDFVTPMVFNTEPHDFDTCFASFKRIAASLIEKI

Download         Length: 942 bp

>T30004 NC_022270:149794-150735 [Vibrio campbellii ATCC BAA-1116]
ATGAATACACTAAAACAACAATTCCTTGAGGTTTCCGATGCGCTTGAGCTCGGAAACCCAGCCATCATTGAAAAAGATTA
CTGGGTCGTGACTTTACTGGCTGAGCTAGCGAAAGTTTCTCCTGAGCATCATCAAATGGTGTTTTCTGGCGGCACTGCGT
TGGCGAAATCAAACGTTAAAATTCTAAGAATGTCGGAAGATGTGGATATAAAACTCATCCCCAACCCAGCATTTGATGAA
CTGAGTCGAGCGAAAAAGAAAACCGCGAGAAAGGCATGTGTTAACGAGATTGAACAGGCACTCACCCAGTCAGGGCTATT
TAACGTTGAAACGCGTTCTGTGCGAGATGAGTACCGCTACGTCGAGTTTGAACTTAAATACCCGCAGCAGTTTAGTCAAG
CGCCCTGCCTGCGCCCCATCATCAAGTTAGAGTTAATCGAAACCATTCCATTACTGGATGTGGAGTCGCGCAGCATTCAA
TCTCTGGTAGCAGAGTTGTACCAACAACCACAAGAAGTGAGTGCATTTGATTGCGTCTCTATCCATGCCACATTGGTTGA
AAAAATTATTTCAATGCTGCGCCGTACCATGTCGGTAAAACGCAATACGGAGCGAAGCGATGATGTTGCTTTAGTTCGCC
ATATTTACGATGTTCACTGCATAACAAAGCTCGCAATGCCAGAACTCAACGAGATAGATGGCCTATTCAAAGCGGTATTG
GCAGAAGATATTGCCCGCTTTGGCAATCAACATGCCGAGTTTGTTGCCAACCCCAAACAAGAGCTACAACTTGGCCTTAA
AGAGCTTGAGGAAAATGCAATATTCCGCCAACGATTTCAGGACTTCGTCACGCCAATGGTGTTTAACACCGAACCCCATG
ATTTTGATACCTGCTTTGCCTCATTTAAACGCATTGCAGCATCGCTAATTGAAAAAATTTAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 15200.53 Da        Isoelectric Point: 10.5319

>AT30004 WP_021018080.1 NC_022270:149388-149792 [Vibrio campbellii ATCC BAA-1116]
MTAADRIYQKIKRSRRYVFERKDFDGFASYDQIGRALRQLVQQGELMKLGYGLYTKARINSLTGKPMPTNPGGTDALMRE
ILKMKGVDFEMDSLSLQSLSGTSTQIPSSIQYSWDPKQFNRKLVVGSRVLNLPS

Download         Length: 405 bp

>AT30004 NC_022270:149388-149792 [Vibrio campbellii ATCC BAA-1116]
ATGACAGCAGCAGACCGCATTTATCAAAAGATAAAGCGTTCAAGACGTTATGTATTTGAACGCAAAGACTTTGATGGATT
TGCAAGTTACGACCAAATAGGTCGGGCCCTGAGGCAGCTTGTGCAGCAAGGTGAGTTAATGAAGCTTGGCTATGGCTTGT
ATACCAAAGCAAGAATCAATAGCCTCACTGGCAAGCCCATGCCGACTAATCCGGGAGGGACCGATGCCCTTATGCGAGAA
ATTCTTAAAATGAAAGGCGTTGATTTTGAGATGGATAGTCTATCGCTGCAAAGTCTTTCAGGCACGAGTACCCAAATTCC
ATCTTCTATTCAGTATTCATGGGACCCAAAACAGTTTAATCGCAAACTGGTGGTCGGTAGCCGTGTGCTGAACTTACCAA
GTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A7N7V0

Antitoxin

Source ID Structure
AlphaFold DB A0A1B1NML2

References