Detailed information of TA system TA029999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 1801651..1802202
• Replicon: chromosome
• Accession: NC_022246
• Organism: Streptococcus intermedius B196

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T1ZH38
• Locus tag: SIR_RS18405
• Protein ID: WP_003041871.1
• Coordinates: 1801924..1802202 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1ZGU4
• Locus tag: SIR_RS18400
• Protein ID: WP_014619943.1
• Coordinates: 1801651..1801923 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SIR_RS18365	1797095..1797498	+	404	Protein_1672	pyridoxamine 5'-phosphate oxidase family protein	-
SIR_RS18370	1797539..1797844	-	306	WP_003073925.1	helix-turn-helix transcriptional regulator	-
SIR_RS18375	1797957..1798418	+	462	Protein_1674	tyrosine-type recombinase/integrase	-
SIR_RS18380	1798711..1799073	+	363	WP_041787576.1	hypothetical protein	-
SIR_RS18385	1799093..1799944	-	852	WP_021003275.1	ImmA/IrrE family metallo-endopeptidase	-
SIR_RS18390	1799951..1800313	-	363	WP_002270986.1	helix-turn-helix domain-containing protein	-
SIR_RS18395	1800956..1801423	-	468	WP_021003276.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
SIR_RS18400	1801651..1801923	+	273	WP_014619943.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SIR_RS18405	1801924..1802202	+	279	WP_003041871.1	type II toxin-antitoxin system YafQ family toxin	Toxin
SIR_RS18410	1802331..1802567	+	237	WP_041787583.1	hypothetical protein	-
SIR_RS18415	1802564..1802791	+	228	WP_041787585.1	hypothetical protein	-
SIR_RS18420	1802905..1803219	+	315	WP_000406626.1	hypothetical protein	-
SIR_RS18425	1803242..1803652	+	411	WP_021003277.1	DUF961 family protein	-
SIR_RS18430	1803659..1805326	+	1668	WP_021003278.1	cell division protein FtsK	-
SIR_RS18435	1805538..1806770	+	1233	WP_021003279.1	replication initiation factor domain-containing protein	-
SIR_RS18440	1806748..1807134	+	387	WP_021003280.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1798125..1817650	19525

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10894.75 Da
• Isoelectric Point: 8.0384

>T29999 WP_003041871.1 NC_022246:1801924-1802202 [Streptococcus intermedius B196]
MYQVKFTSAFKKSYKRIKKRGLEMALLDEVIEKLRLGQTLEEKYRDHELKGNFVGFRECHIKPDWLLIYLIEDDILTLTL
ADTGSHADLFKM

Nucleotide

• Length: 279 bp

>T29999 NC_022246:1801924-1802202 [Streptococcus intermedius B196]
ATGTATCAAGTCAAGTTCACCTCTGCTTTTAAAAAGAGCTATAAACGGATTAAAAAGCGAGGTTTAGAGATGGCACTCTT
AGATGAAGTGATTGAAAAACTCCGTCTAGGTCAGACACTCGAAGAAAAATACCGTGACCACGAACTCAAAGGAAATTTTG
TTGGCTTTAGGGAATGCCACATCAAGCCAGATTGGCTCTTGATTTATCTGATTGAAGATGATATTTTGACCCTAACCTTA
GCCGATACAGGCAGTCATGCGGATTTGTTTAAGATGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9905.35 Da
• Isoelectric Point: 4.7085

>AT29999 WP_014619943.1 NC_022246:1801651-1801923 [Streptococcus intermedius B196]
MATVPTQIRIDETVKAQATSLFNDLGMDMSSAVNIFLRQCLLRGGLPFTVELPNYSQKTLEALAEAKRISRDETVASYDN
LADLKKALDA

Nucleotide

• Length: 273 bp

>AT29999 NC_022246:1801651-1801923 [Streptococcus intermedius B196]
ATGGCAACTGTACCAACTCAAATTCGGATTGATGAAACTGTAAAAGCCCAAGCAACTTCATTATTTAATGACTTGGGGAT
GGATATGTCTAGTGCAGTCAATATTTTTCTTCGTCAATGTCTATTGCGTGGTGGGCTTCCTTTCACGGTTGAACTTCCCA
ATTATAGTCAAAAGACCTTAGAAGCTCTGGCAGAAGCTAAGCGTATTTCTCGTGATGAAACGGTTGCTTCTTATGACAAT
CTAGCAGATTTAAAGAAAGCTCTGGACGCTTAG

Structures

Toxin

• AlphaFold DB: A0A3R8STB9

Antitoxin

• AlphaFold DB: A0A3R9JSK3