Detailed information of TA system TA029977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2095526..2096040
• Replicon: chromosome
• Accession: NC_022226
• Organism: Staphylococcus aureus subsp. aureus CN1

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: SAKOR_RS10560
• Protein ID: WP_000581792.1
• Coordinates: 2095526..2095735 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: SAKOR_RS10565
• Protein ID: WP_000138932.1
• Coordinates: 2095747..2096040 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAKOR_RS10540	2091034..2092392	-	1359	WP_000611470.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
SAKOR_RS10545	2092407..2093477	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
SAKOR_RS10550	2093795..2094997	+	1203	WP_020978293.1	rod shape-determining protein RodA	-
SAKOR_RS10555	2095261..2095398	-	138	WP_000828354.1	hypothetical protein	-
SAKOR_RS10560	2095526..2095735	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
SAKOR_RS10565	2095747..2096040	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
SAKOR_RS10570	2096206..2097691	+	1486	Protein_1993	cardiolipin synthase	-
SAKOR_RS10575	2097719..2098366	+	648	WP_001187630.1	HD domain-containing protein	-
SAKOR_RS10580	2098838..2099710	-	873	WP_000725802.1	membrane protein insertase YidC	-
SAKOR_RS10585	2099796..2100437	-	642	WP_000483153.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T29977 WP_000581792.1 NC_022226:c2095735-2095526 [Staphylococcus aureus subsp. aureus CN1]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T29977 NC_022226:c2095735-2095526 [Staphylococcus aureus subsp. aureus CN1]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT29977 WP_000138932.1 NC_022226:c2096040-2095747 [Staphylococcus aureus subsp. aureus CN1]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT29977 NC_022226:c2096040-2095747 [Staphylococcus aureus subsp. aureus CN1]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13