Detailed information of TA system TA029976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2075785..2076314
• Replicon: chromosome
• Accession: NC_022226
• Organism: Staphylococcus aureus subsp. aureus CN1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAKOR_RS10470
• Protein ID: WP_000621175.1
• Coordinates: 2075785..2076147 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAKOR_RS10475
• Protein ID: WP_000948331.1
• Coordinates: 2076144..2076314 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAKOR_RS10445	2072764..2073534	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
SAKOR_RS10450	2073509..2073988	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAKOR_RS10455	2073990..2074316	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAKOR_RS10460	2074435..2075436	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAKOR_RS10470	2075785..2076147	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAKOR_RS10475	2076144..2076314	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAKOR_RS10480	2076399..2077547	-	1149	WP_001281142.1	alanine racemase	-
SAKOR_RS10485	2077613..2077972	-	360	WP_000581197.1	holo-ACP synthase	-
SAKOR_RS10490	2077976..2078467	-	492	WP_001205910.1	PH domain-containing protein	-
SAKOR_RS10495	2078454..2080037	-	1584	WP_001294626.1	PH domain-containing protein	-
SAKOR_RS10500	2080030..2080509	-	480	WP_001287090.1	hypothetical protein	-
SAKOR_RS10505	2080718..2081278	-	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T29976 WP_000621175.1 NC_022226:c2076147-2075785 [Staphylococcus aureus subsp. aureus CN1]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T29976 NC_022226:c2076147-2075785 [Staphylococcus aureus subsp. aureus CN1]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT29976 WP_000948331.1 NC_022226:c2076314-2076144 [Staphylococcus aureus subsp. aureus CN1]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT29976 NC_022226:c2076314-2076144 [Staphylococcus aureus subsp. aureus CN1]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1