Detailed information of TA system TA029965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 9671..10437
• Replicon: chromosome
• Accession: NC_022224
• Organism: Listonella anguillarum M3

Toxin (Protein)

• Gene name: -
• Uniprot ID: F7YRS3
• Locus tag: N175_RS14060
• Protein ID: WP_013867769.1
• Coordinates: 9671..10168 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: F7YRS4
• Locus tag: N175_RS14065
• Protein ID: WP_013867770.1
• Coordinates: 10165..10437 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N175_RS14035	6140..6493	-	354	WP_011154649.1	IS66 family insertion sequence element accessory protein TnpB	-
N175_RS14040	6490..6807	-	318	WP_011154648.1	IS66 family insertion sequence hypothetical protein	-
N175_RS18995	6922..7014	+	93	WP_194291283.1	DUF3265 domain-containing protein	-
N175_RS19000	7102..7308	+	207	WP_013867765.1	DUF1289 domain-containing protein	-
N175_RS14045	7428..7658	-	231	WP_013867766.1	PAS factor family protein	-
N175_RS14050	7952..8188	-	237	WP_013867767.1	hypothetical protein	-
N175_RS14055	8473..8961	+	489	WP_013867768.1	hypothetical protein	-
N175_RS19315	8952..9083	+	132	Protein_14	DUF3265 domain-containing protein	-
N175_RS14060	9671..10168	-	498	WP_013867769.1	GNAT family N-acetyltransferase	Toxin
N175_RS14065	10165..10437	-	273	WP_013867770.1	DUF1778 domain-containing protein	Antitoxin
N175_RS14075	11188..11907	+	720	Protein_17	hypothetical protein	-
N175_RS14080	11964..12884	-	921	WP_011154643.1	IS5-like element ISVa2 family transposase	-
N175_RS14085	12983..13276	+	294	WP_020977843.1	potassium channel family protein	-
N175_RS14090	13549..13938	+	390	WP_019280976.1	DUF1090 domain-containing protein	-
N175_RS19010	13942..14031	+	90	WP_019280977.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	768..13938	13170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18525.25 Da
• Isoelectric Point: 9.2555

>T29965 WP_013867769.1 NC_022224:c10168-9671 [Vibrio anguillarum M3]
MMNTVLLDKVKHDRNRFNCGIEALNNYLKVMASQQAKKDNTRTFVLEDDNNSSHIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKYFYERYGFQAFQDAENKLFITIADV
RASLG

Nucleotide

• Length: 498 bp

>T29965 NC_022224:c10168-9671 [Vibrio anguillarum M3]
ATGATGAATACGGTACTTTTAGATAAAGTTAAACACGATAGAAACCGATTCAACTGTGGTATCGAAGCTCTCAATAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCAAAGAAAGACAACACCAGAACGTTTGTTCTGGAAGATGATAACAACAGCTCAC
ATATCATCGGCTTTTACACGCTAACAATGACACCAATTGACTTAAAGGCATTGCCCGATAAATTACAAAAAAAGCACCAA
TCATCCACCTCTGGTGGCCTAATTGCCCGTCTTGCTGTCGATGACAGATACAAAGGGAAAGGCTTTGGTGAGTGGCTGCT
CATCGATGCACTCAGAAAGCTATTAGCTGCTAGTGATAGCGTTGCATTTCCAGTTGTTATCGTTGATGCCAAAGACGGTG
CAAAATATTTTTATGAACGCTATGGCTTTCAGGCATTTCAAGACGCTGAAAACAAACTCTTCATCACCATTGCGGATGTA
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9731.18 Da
• Isoelectric Point: 8.6154

>AT29965 WP_013867770.1 NC_022224:c10437-10165 [Vibrio anguillarum M3]
MATTLPRITARVDVDTQDLLTKAAAIAGMSSINSFVLSAAIEKAKQVIEREQALKLSQADAILLMDALDRPTAQNSKLRA
ASERYESKAQ

Nucleotide

• Length: 273 bp

>AT29965 NC_022224:c10437-10165 [Vibrio anguillarum M3]
ATGGCTACTACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACACAAGATTTACTCACTAAAGCTGCCGCTATCGC
TGGTATGTCTAGTATTAACTCATTTGTTCTAAGCGCTGCAATTGAAAAGGCTAAACAAGTCATCGAACGTGAGCAAGCTT
TAAAACTGAGCCAAGCCGATGCGATTTTGCTGATGGACGCATTAGACCGCCCAACAGCACAGAACTCAAAACTAAGAGCG
GCCTCTGAACGATACGAGAGCAAGGCTCAATGA

Structures

Toxin

• AlphaFold DB: A0A7Y3QZT4

Antitoxin

• AlphaFold DB: A0A7X8U164