Detailed information of TA system TA029958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2059407..2059921
• Replicon: chromosome
• Accession: NC_022222
• Organism: Staphylococcus aureus subsp. aureus 6850

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: RSAU_RS10290
• Protein ID: WP_000581792.1
• Coordinates: 2059407..2059616 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: RSAU_RS10295
• Protein ID: WP_000138934.1
• Coordinates: 2059628..2059921 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSAU_RS10270	2054914..2056272	-	1359	WP_000611467.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
RSAU_RS10275	2056287..2057357	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
RSAU_RS10280	2057675..2058877	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
RSAU_RS10285	2059141..2059278	-	138	WP_000828354.1	hypothetical protein	-
RSAU_RS10290	2059407..2059616	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
RSAU_RS10295	2059628..2059921	-	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
RSAU_RS10300	2060087..2061571	+	1485	WP_020977447.1	cardiolipin synthase	-
RSAU_RS10305	2061599..2062246	+	648	WP_001187619.1	HD domain-containing protein	-
RSAU_RS10315	2063060..2063932	-	873	WP_000725802.1	membrane protein insertase YidC	-
RSAU_RS10320	2064018..2064659	-	642	WP_000483165.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T29958 WP_000581792.1 NC_022222:c2059616-2059407 [Staphylococcus aureus subsp. aureus 6850]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T29958 NC_022222:c2059616-2059407 [Staphylococcus aureus subsp. aureus 6850]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT29958 WP_000138934.1 NC_022222:c2059921-2059628 [Staphylococcus aureus subsp. aureus 6850]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT29958 NC_022222:c2059921-2059628 [Staphylococcus aureus subsp. aureus 6850]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: A0A0D1GBN9