Detailed information of TA system TA029957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2039663..2040192
• Replicon: chromosome
• Accession: NC_022222
• Organism: Staphylococcus aureus subsp. aureus 6850

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RSAU_RS10200
• Protein ID: WP_000621175.1
• Coordinates: 2039663..2040025 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RSAU_RS10205
• Protein ID: WP_000948331.1
• Coordinates: 2040022..2040192 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSAU_RS10175	2036641..2037411	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
RSAU_RS10180	2037386..2037865	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
RSAU_RS10185	2037867..2038193	-	327	WP_020977444.1	anti-sigma factor antagonist	-
RSAU_RS10190	2038312..2039313	-	1002	WP_020977445.1	PP2C family protein-serine/threonine phosphatase	-
RSAU_RS10200	2039663..2040025	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RSAU_RS10205	2040022..2040192	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RSAU_RS10210	2040277..2041425	-	1149	WP_001281148.1	alanine racemase	-
RSAU_RS10215	2041491..2041850	-	360	WP_000581197.1	holo-ACP synthase	-
RSAU_RS10220	2041854..2042345	-	492	WP_001205916.1	PH domain-containing protein	-
RSAU_RS10225	2042332..2043915	-	1584	WP_001294642.1	PH domain-containing protein	-
RSAU_RS10230	2043908..2044387	-	480	WP_001287083.1	hypothetical protein	-
RSAU_RS10235	2044596..2045156	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T29957 WP_000621175.1 NC_022222:c2040025-2039663 [Staphylococcus aureus subsp. aureus 6850]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T29957 NC_022222:c2040025-2039663 [Staphylococcus aureus subsp. aureus 6850]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT29957 WP_000948331.1 NC_022222:c2040192-2040022 [Staphylococcus aureus subsp. aureus 6850]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT29957 NC_022222:c2040192-2040022 [Staphylococcus aureus subsp. aureus 6850]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1