Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2535077..2535728 Replicon chromosome
Accession NC_022112
Organism Lactobacillus paracasei subsp. paracasei 8700:2

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag LBPG_RS12355 Protein ID WP_003567661.1
Coordinates 2535077..2535460 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A826I0C1
Locus tag LBPG_RS12360 Protein ID WP_003658732.1
Coordinates 2535480..2535728 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBPG_RS12350 2534372..2534893 - 522 WP_003567657.1 QueT transporter family protein -
LBPG_RS12355 2535077..2535460 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LBPG_RS12360 2535480..2535728 - 249 WP_003658732.1 hypothetical protein Antitoxin
LBPG_RS12365 2535796..2536932 - 1137 WP_003658734.1 alanine racemase -
LBPG_RS12370 2536919..2537293 - 375 WP_003567669.1 holo-ACP synthase -
LBPG_RS12375 2537462..2538970 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
LBPG_RS16255 2539096..2539239 - 144 WP_003567673.1 hypothetical protein -
LBPG_RS12385 2539270..2540658 - 1389 WP_003567677.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T29922 WP_003567661.1 NC_022112:c2535460-2535077 [Lactobacillus paracasei subsp. paracasei 8700:2]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T29922 NC_022112:c2535460-2535077 [Lactobacillus paracasei subsp. paracasei 8700:2]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCGGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGCTTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8866.02 Da        Isoelectric Point: 4.6250

>AT29922 WP_003658732.1 NC_022112:c2535728-2535480 [Lactobacillus paracasei subsp. paracasei 8700:2]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITGAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT29922 NC_022112:c2535728-2535480 [Lactobacillus paracasei subsp. paracasei 8700:2]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCTGTGATTACGGGGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A826I0C1

References