Detailed information of TA system TA029918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 1587207..1587846
• Replicon: chromosome
• Accession: NC_022093
• Organism: Thermofilum sp. 1910b

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: N186_RS08545
• Protein ID: WP_052885580.1
• Coordinates: 1587207..1587623 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: S5ZXM9
• Locus tag: N186_RS08550
• Protein ID: WP_020963416.1
• Coordinates: 1587604..1587846 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N186_RS08525 (N186_08840)	1582979..1583779	-	801	WP_020963411.1	hypothetical protein	-
N186_RS08530 (N186_08845)	1583804..1585081	-	1278	WP_020963412.1	APC family permease	-
N186_RS08535 (N186_08850)	1585229..1585615	+	387	WP_020963413.1	hypothetical protein	-
N186_RS08540 (N186_08855)	1585663..1587033	+	1371	WP_020963414.1	alpha-glucosidase/alpha-galactosidase	-
N186_RS08545 (N186_08860)	1587207..1587623	-	417	WP_052885580.1	PIN domain-containing protein	Toxin
N186_RS08550 (N186_08865)	1587604..1587846	-	243	WP_020963416.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
N186_RS08555 (N186_08870)	1588226..1588588	-	363	WP_020963417.1	hypothetical protein	-
N186_RS08560 (N186_08875)	1588689..1589567	+	879	WP_020963418.1	helix-turn-helix domain-containing protein	-
N186_RS08565 (N186_08880)	1589696..1590250	+	555	WP_020963419.1	hypothetical protein	-
N186_RS08570 (N186_08885)	1590319..1591770	+	1452	WP_020963420.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• SpoVT-AbrB domain (5-46)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15776.21 Da
• Isoelectric Point: 4.6474

>T29918 WP_052885580.1 NC_022093:c1587623-1587207 [Thermofilum adornatum]
MPEAVIDTNILVYDTFEDSLYHKEASHILDSLNRWIIPLIVIYEYAWFLKGLKMSTADARDKVLEYITSEKSVLVREGLD
EIRWAISTLVDEGLSLSCFNDKIFLSVALRRNVPLATFDEKLRRQASNIGVALVPETL

Nucleotide

• Length: 417 bp

>T29918 NC_022093:c1587623-1587207 [Thermofilum adornatum]
TTGCCGGAGGCGGTGATTGACACAAATATCCTTGTCTACGATACCTTTGAGGATTCTCTCTACCACAAGGAGGCTTCCCA
TATACTTGACAGTTTAAACCGGTGGATAATCCCCCTAATAGTCATCTACGAGTATGCTTGGTTCCTGAAGGGGTTAAAAA
TGAGTACAGCTGATGCTCGGGACAAGGTACTCGAGTATATTACCTCCGAGAAGTCCGTATTGGTGAGGGAGGGCTTAGAT
GAGATCCGCTGGGCTATCTCTACACTTGTAGATGAGGGCCTGTCTCTCTCATGCTTCAACGACAAAATCTTTCTTTCAGT
AGCTCTGCGAAGAAATGTTCCACTAGCGACCTTCGATGAAAAGCTGAGGAGGCAAGCATCCAATATAGGTGTGGCATTAG
TTCCCGAGACATTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8929.44 Da
• Isoelectric Point: 10.2100

>AT29918 WP_020963416.1 NC_022093:c1587846-1587604 [Thermofilum adornatum]
VKRSKITRSFQVTIPKEIREALGLQIGDYLDMYVDEKGRIIMEKKVLKRKTFRAGRPLTPEEIDGIIAKGLGENIAGGGD

Nucleotide

• Length: 243 bp

>AT29918 NC_022093:c1587846-1587604 [Thermofilum adornatum]
GTGAAGAGAAGTAAAATAACGAGGAGTTTTCAAGTAACGATTCCAAAAGAGATACGTGAAGCATTGGGACTGCAGATCGG
CGACTACCTGGACATGTATGTGGATGAAAAGGGGAGAATCATTATGGAGAAAAAAGTTCTGAAGAGGAAGACTTTTAGAG
CTGGAAGACCCCTTACTCCGGAAGAAATTGATGGGATAATCGCTAAAGGGCTGGGTGAAAACATTGCCGGAGGCGGTGAT
TGA

Structures

Antitoxin

• AlphaFold DB: S5ZXM9