Detailed information of TA system TA029914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 490962..491599
• Accession: NC_022081
• Organism: Bacillus amyloliquefaciens subsp. plantarum UCMB5113

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BASU_RS02800
• Protein ID: WP_003156187.1
• Coordinates: 491249..491599 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BASU_RS02795
• Protein ID: WP_003156188.1
• Coordinates: 490962..491243 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BASU_RS02775	487327..487926	-	600	WP_020955355.1	rhomboid family intramembrane serine protease	-
BASU_RS02780	488019..488384	+	366	WP_007410229.1	holo-ACP synthase	-
BASU_RS02785	488549..489556	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
BASU_RS02790	489673..490842	+	1170	WP_020955356.1	alanine racemase	-
BASU_RS02795	490962..491243	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BASU_RS02800	491249..491599	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BASU_RS02805	491717..492538	+	822	WP_003156182.1	STAS domain-containing protein	-
BASU_RS02810	492543..492908	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BASU_RS02815	492911..493312	+	402	WP_003156178.1	anti-sigma regulatory factor	-
BASU_RS02820	493324..494331	+	1008	WP_020955357.1	PP2C family protein-serine/threonine phosphatase	-
BASU_RS02825	494395..494724	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BASU_RS02830	494721..495203	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
BASU_RS02835	495169..495957	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
BASU_RS02840	495957..496559	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T29914 WP_003156187.1 NC_022081:491249-491599 [Bacillus velezensis UCMB5113]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T29914 NC_022081:491249-491599 [Bacillus velezensis UCMB5113]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT29914 WP_003156188.1 NC_022081:490962-491243 [Bacillus velezensis UCMB5113]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT29914 NC_022081:490962-491243 [Bacillus velezensis UCMB5113]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5