Detailed information of TA system TA029913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): doc-NpoA/Fic(toxin)
• Location: 633732..635090
• Replicon: chromosome
• Accession: NC_022080
• Organism: Geobacillus sp. JF8

Toxin (Protein)

• Gene name: doc
• Uniprot ID: S5Z238
• Locus tag: M493_RS03320
• Protein ID: WP_020958857.1
• Coordinates: 634698..635090 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: NpoA
• Uniprot ID: S6A0C8
• Locus tag: M493_RS03315
• Protein ID: WP_020958856.1
• Coordinates: 633732..634595 (-)
• Length: 288 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M493_RS03300	630001..630843	+	843	WP_020958853.1	sugar ABC transporter permease	-
M493_RS03305	630914..632449	+	1536	WP_020958854.1	alpha-amylase	-
M493_RS03310	632468..633493	+	1026	WP_020958855.1	LacI family DNA-binding transcriptional regulator	-
M493_RS03315	633732..634595	-	864	WP_020958856.1	SDR family oxidoreductase	Antitoxin
M493_RS03320	634698..635090	-	393	WP_020958857.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
M493_RS03325	635103..635375	-	273	WP_020958858.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
M493_RS03330	635813..639223	-	3411	WP_020958859.1	methionine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14957.19 Da
• Isoelectric Point: 9.3388

>T29913 WP_020958857.1 NC_022080:c635090-634698 [Geobacillus genomosp. 3]
MTRFLTEKEIIFLNALLIEKYTPGEQKGVKSPELLNSAVNRPKQSAFGQDAYPTLWLKAAALYASLCQNHPFHNANKRTG
FAAMKQFLWLNGYRFAASEKEAEDYTVKVVTDKPSIEEIAMWIERWAEKR

Nucleotide

• Length: 393 bp

>T29913 NC_022080:c635090-634698 [Geobacillus genomosp. 3]
ATGACTCGTTTCTTGACGGAAAAAGAAATTATCTTCCTCAATGCCTTGTTGATCGAAAAATACACTCCAGGGGAACAAAA
AGGGGTGAAATCTCCAGAGTTGTTGAACAGCGCGGTCAACCGCCCGAAACAAAGCGCTTTCGGACAGGACGCTTATCCAA
CTCTTTGGCTGAAAGCGGCAGCGTTGTACGCCAGCCTTTGCCAAAACCATCCGTTTCATAACGCCAATAAACGAACAGGA
TTTGCCGCCATGAAACAGTTCCTTTGGTTAAACGGTTATCGCTTTGCCGCTTCAGAAAAAGAGGCAGAGGACTATACCGT
GAAAGTCGTGACCGATAAACCATCAATTGAAGAAATTGCGATGTGGATTGAAAGATGGGCGGAAAAAAGATGA

Antitoxin

Protein

• Length: 288 a.a.
• Molecular weight: 30806.95 Da
• Isoelectric Point: 6.4921

>AT29913 WP_020958856.1 NC_022080:c634595-633732 [Geobacillus genomosp. 3]
MVTKQQTTLPPQHQTRQPGLQTEMNPQPVTIKDTYQGSGKLENRVAIISGGDSGIGRAVAVHFAKEGADVAIVYLNEHED
AEETKRLVEQEGRRCLAIAGDIGDEAFCKEAVKQTIEAFGKLDIVVNNAAEQHPQPNFLNITAAQLEKTFRTNVFGCFFL
TKAALPHLKSGSAIINTASITAYEGNEQLIDYSATKGAIVAFTRSLAKALVGQGIRVNGVAPGPIWTPLIPSTFKSEQVA
TFGANTPMKRPGQPSEVAPCYVFLASDESSYMTGQMLHVDGGKFVSG

Nucleotide

• Length: 864 bp

>AT29913 NC_022080:c634595-633732 [Geobacillus genomosp. 3]
ATGGTTACGAAGCAACAAACGACCTTGCCCCCGCAGCACCAAACGCGTCAGCCGGGCCTGCAAACGGAGATGAACCCGCA
GCCGGTCACCATCAAAGACACGTATCAAGGAAGCGGCAAGCTCGAAAACAGAGTCGCCATCATCAGCGGCGGCGACAGCG
GCATCGGCCGGGCGGTCGCCGTTCATTTCGCCAAAGAAGGAGCCGATGTGGCGATTGTTTATCTCAATGAACATGAAGAC
GCTGAGGAAACGAAACGGCTCGTCGAACAGGAAGGAAGACGGTGCCTGGCCATTGCGGGGGACATCGGAGATGAAGCGTT
TTGCAAAGAAGCGGTGAAACAGACGATCGAAGCGTTCGGCAAGCTTGACATCGTCGTCAACAACGCCGCCGAACAGCACC
CGCAGCCGAACTTTCTCAACATCACCGCCGCACAGCTGGAAAAAACGTTCCGCACGAACGTGTTCGGCTGCTTTTTCTTA
ACGAAAGCGGCGCTCCCGCACTTAAAAAGCGGAAGCGCCATTATTAACACCGCCTCGATCACCGCTTATGAAGGAAACGA
GCAATTGATCGACTACTCGGCGACAAAAGGGGCGATTGTCGCGTTCACCCGCTCGCTCGCCAAAGCGCTCGTCGGCCAAG
GCATTCGCGTCAACGGCGTCGCCCCGGGGCCCATTTGGACGCCGCTCATCCCGTCGACATTCAAAAGCGAGCAAGTCGCC
ACATTCGGCGCCAACACGCCGATGAAACGGCCCGGCCAGCCGAGCGAGGTCGCCCCGTGCTACGTCTTTTTGGCCAGCGA
CGAATCGTCGTACATGACCGGGCAAATGCTTCACGTCGACGGAGGAAAGTTCGTCAGCGGATGA

Structures

Toxin

• AlphaFold DB: S5Z238

Antitoxin

• AlphaFold DB: S6A0C8