Detailed information of TA system TA029911

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1268470..1269387
• Accession: NC_022075
• Organism: Bacillus amyloliquefaciens subsp. plantarum UCMB5033

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: RBAU_RS06430
• Protein ID: WP_007407256.1
• Coordinates: 1268641..1269387 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: RBAU_RS06425
• Protein ID: WP_003154807.1
• Coordinates: 1268470..1268640 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RBAU_RS06390	1263704..1265326	+	1623	WP_007407261.1	pyocin knob domain-containing protein	-
RBAU_RS06395	1265339..1265710	+	372	WP_007407260.1	hypothetical protein	-
RBAU_RS06400	1265715..1265912	+	198	WP_007407259.1	XkdX family protein	-
RBAU_RS06405	1265969..1266730	+	762	WP_007407258.1	hypothetical protein	-
RBAU_RS06410	1266782..1267045	+	264	WP_003154815.1	hemolysin XhlA family protein	-
RBAU_RS06415	1267059..1267322	+	264	WP_003154813.1	phage holin	-
RBAU_RS06420	1267336..1268214	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
RBAU_RS06425	1268470..1268640	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
RBAU_RS06430	1268641..1269387	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
RBAU_RS06435	1269492..1270490	-	999	WP_007407255.1	inorganic phosphate transporter	-
RBAU_RS06440	1270503..1271120	-	618	WP_003154804.1	DUF47 domain-containing protein	-
RBAU_RS06445	1271406..1272722	-	1317	WP_007407254.1	amino acid permease	-
RBAU_RS06450	1273045..1273995	+	951	WP_007407253.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T29911 WP_007407256.1 NC_022075:c1269387-1268641 [Bacillus velezensis UCMB5033]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T29911 NC_022075:c1269387-1268641 [Bacillus velezensis UCMB5033]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGATTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT29911 WP_003154807.1 NC_022075:c1268640-1268470 [Bacillus velezensis UCMB5033]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT29911 NC_022075:c1268640-1268470 [Bacillus velezensis UCMB5033]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14