Detailed information of TA system TA029909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 42052..42629
• Replicon: plasmid unnamed
• Accession: NC_022045
• Organism: Listeria monocytogenes

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: HK93_RS15855
• Protein ID: WP_003728465.1
• Coordinates: 42288..42629 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: HK93_RS15850
• Protein ID: WP_003728464.1
• Coordinates: 42052..42294 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HK93_RS15805	37324..38040	-	717	WP_003731678.1	AP2 domain-containing protein	-
HK93_RS15810	38056..38778	-	723	WP_003728517.1	CRISPR-associated protein Cas5	-
HK93_RS15815	38782..39207	-	426	WP_003728518.1	hypothetical protein	-
HK93_RS15820	39208..39456	-	249	WP_003731677.1	hypothetical protein	-
HK93_RS15825	39578..40066	-	489	WP_003728520.1	hypothetical protein	-
HK93_RS15830	40225..40602	-	378	WP_003769263.1	DUF3850 domain-containing protein	-
HK93_RS15835	40621..41283	-	663	WP_003731676.1	DUF2786 domain-containing protein	-
HK93_RS15840	41283..41609	-	327	WP_003728462.1	hypothetical protein	-
HK93_RS15845	41740..41991	+	252	WP_003728463.1	hypothetical protein	-
HK93_RS15850	42052..42294	+	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HK93_RS15855	42288..42629	+	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HK93_RS15860	42822..44957	-	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
HK93_RS15865	44957..45316	-	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
HK93_RS15870	45596..46150	+	555	WP_003728468.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..148959	148959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (12-128)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 14629.86 Da
• Isoelectric Point: 12.8531

>T29909 WP_003728465.1 NC_022045:42288-42629 [Listeria monocytogenes]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 342 bp

>T29909 NC_022045:42288-42629 [Listeria monocytogenes]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 10400.12 Da
• Isoelectric Point: 12.7030

>AT29909 WP_003728464.1 NC_022045:42052-42294 [Listeria monocytogenes]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
K

Nucleotide

• Length: 243 bp

>AT29909 NC_022045:42052-42294 [Listeria monocytogenes]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAA

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5