Detailed information of TA system TA029903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 48291..48941
• Replicon: plasmid p2
• Accession: NC_021995
• Organism: Enterococcus faecium Aus0085

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EFAU085_RS15700
• Protein ID: WP_002332741.1
• Coordinates: 48291..48476 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A829EWE8
• Locus tag: EFAU085_RS15705
• Protein ID: WP_002305052.1
• Coordinates: 48510..48941 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFAU085_RS15655 (EFAU085_p2048)	43598..44497	+	900	WP_014401509.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
EFAU085_RS15660 (EFAU085_p2049)	44500..44985	+	486	WP_014401510.1	single-stranded DNA-binding protein	-
EFAU085_RS15665 (EFAU085_p2050)	45351..45611	+	261	WP_002296240.1	hypothetical protein	-
EFAU085_RS15670	46058..46264	+	207	WP_002296239.1	hypothetical protein	-
EFAU085_RS15675 (EFAU085_p2051)	46264..46488	+	225	WP_020944759.1	hypothetical protein	-
EFAU085_RS15680 (EFAU085_p2052)	46503..46940	+	438	WP_020944760.1	hypothetical protein	-
EFAU085_RS15685 (EFAU085_p2053)	46933..47640	+	708	WP_020944761.1	hypothetical protein	-
EFAU085_RS18245	47803..48003	+	201	WP_002330566.1	hypothetical protein	-
EFAU085_RS15695 (EFAU085_p2054)	48019..48198	+	180	WP_002295613.1	hypothetical protein	-
EFAU085_RS15700 (EFAU085_p2055)	48291..48476	+	186	WP_002332741.1	type II toxin-antitoxin system HicA family toxin	Toxin
EFAU085_RS15705 (EFAU085_p2056)	48510..48941	+	432	WP_002305052.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EFAU085_RS15710 (EFAU085_p2058)	49879..51153	+	1275	WP_002323715.1	MFS transporter	-
EFAU085_RS15715 (EFAU085_p2059)	51207..52634	+	1428	WP_002332665.1	sucrose-6-phosphate hydrolase	-
EFAU085_RS15720 (EFAU085_p2060)	52647..53519	+	873	WP_020944763.1	ROK family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67314	67314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6969.15 Da
• Isoelectric Point: 10.6196

>T29903 WP_002332741.1 NC_021995:48291-48476 [Enterococcus faecium Aus0085]
MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQDLGRGLEQKILKDAGLK

Nucleotide

• Length: 186 bp

>T29903 NC_021995:48291-48476 [Enterococcus faecium Aus0085]
ATGCCACTTACGGGAAAAGAAATGCTTAAGTTATTGAAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATCTCATCA
TCATCTTTATAAGGATGGTGTTAGAATCACTGTTCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCAAAAAA
TTTTAAAGGATGCGGGATTGAAATAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15811.92 Da
• Isoelectric Point: 4.1638

>AT29903 WP_002305052.1 NC_021995:48510-48941 [Enterococcus faecium Aus0085]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT29903 NC_021995:48510-48941 [Enterococcus faecium Aus0085]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGAATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGATCTACTTCCAGAACCAACACCAATCAATAAGATTTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGCTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Structures

Antitoxin

• AlphaFold DB: A0A829EWE8