Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 465639..466210 Replicon chromosome
Accession NC_021994
Organism Enterococcus faecium Aus0085

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag EFAU085_RS02200 Protein ID WP_002286801.1
Coordinates 465869..466210 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag EFAU085_RS02195 Protein ID WP_002323011.1
Coordinates 465639..465869 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFAU085_RS02170 (EFAU085_00443) 460882..462213 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
EFAU085_RS02175 (EFAU085_00444) 462235..462861 + 627 WP_002333420.1 cysteine hydrolase -
EFAU085_RS02180 (EFAU085_00445) 463044..463625 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
EFAU085_RS02185 (EFAU085_00446) 464234..464809 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
EFAU085_RS02190 (EFAU085_00447) 465014..465352 - 339 WP_002286804.1 hypothetical protein -
EFAU085_RS02195 (EFAU085_00448) 465639..465869 + 231 WP_002323011.1 hypothetical protein Antitoxin
EFAU085_RS02200 (EFAU085_00449) 465869..466210 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EFAU085_RS02205 (EFAU085_00450) 467060..467245 + 186 WP_002304207.1 hypothetical protein -
EFAU085_RS02210 (EFAU085_00451) 467750..467944 + 195 WP_002295146.1 hypothetical protein -
EFAU085_RS16290 468135..468384 + 250 Protein_441 transposase -
EFAU085_RS02220 (EFAU085_00452) 468628..469458 - 831 WP_002286789.1 manganese catalase family protein -
EFAU085_RS02225 (EFAU085_00453) 469666..471000 + 1335 WP_002333419.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T29902 WP_002286801.1 NC_021994:465869-466210 [Enterococcus faecium Aus0085]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T29902 NC_021994:465869-466210 [Enterococcus faecium Aus0085]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT29902 WP_002323011.1 NC_021994:465639-465869 [Enterococcus faecium Aus0085]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT29902 NC_021994:465639-465869 [Enterococcus faecium Aus0085]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References