29900

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 12696..13804 Replicon plasmid p3
Accession NC_021988
Organism Enterococcus faecium Aus0085

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag EFAU085_RS15895 Protein ID WP_002303392.1
Coordinates 12696..13565 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag EFAU085_RS15900 Protein ID WP_002303393.1
Coordinates 13580..13804 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFAU085_RS15865 8002..8085 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
EFAU085_RS15870 (EFAU085_p3013) 8627..9421 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EFAU085_RS17310 9514..9765 - 252 Protein_13 GNAT family N-acetyltransferase -
EFAU085_RS15875 (EFAU085_p3014) 9884..10792 + 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
EFAU085_RS15880 (EFAU085_p3015) 10816..11043 - 228 Protein_15 hypothetical protein -
EFAU085_RS15885 (EFAU085_p3016) 11040..11948 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EFAU085_RS15890 (EFAU085_p3017) 11981..12715 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EFAU085_RS15895 (EFAU085_p3018) 12696..13565 - 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
EFAU085_RS15900 (EFAU085_p3019) 13580..13804 - 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
EFAU085_RS15905 (EFAU085_p3020) 13947..14768 - 822 Protein_20 recombinase zinc beta ribbon domain-containing protein -
EFAU085_RS15910 (EFAU085_p3022) 15456..16257 - 802 Protein_21 lincosamide nucleotidyltransferase Lnu(B) -
EFAU085_RS15915 (EFAU085_p3023) 16311..17795 - 1485 WP_020945028.1 ABC-F type ribosomal protection protein Lsa(E) -
EFAU085_RS15920 (EFAU085_p3024) 18238..18606 - 369 WP_060854318.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 1..31004 31004
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 1880..28310 26430


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T29900 WP_002303392.1 NC_021988:c13565-12696 [Enterococcus faecium Aus0085]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T29900 NC_021988:c13565-12696 [Enterococcus faecium Aus0085]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATTGTATTAGGGGGCTC
ACGTGCAAGAAGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAGTTGGATGATGAGAATAGAAACAACCTTGTTGTACCTCCCGGAGCGTGGGGTGATTGG
GTTAATGGCGGTGGATGGTTAGTTATAAACGGGTGTCATGTTGACTTGATATTACGTGATATAAAACGGGTGGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCTATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGCAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGACATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT29900 WP_002303393.1 NC_021988:c13804-13580 [Enterococcus faecium Aus0085]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp

>AT29900 NC_021988:c13804-13580 [Enterococcus faecium Aus0085]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTTAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGATGAAATAAAAATATCTAACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References