Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 5740..6877 Replicon plasmid p3
Accession NC_021988
Organism Enterococcus faecium Aus0085

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag EFAU085_RS15855 Protein ID WP_001284311.1
Coordinates 6014..6877 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag EFAU085_RS15850 Protein ID WP_000301765.1
Coordinates 5740..6012 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFAU085_RS15820 (EFAU085_p3002) 1560..1856 + 297 WP_000053907.1 replication control protein PrgN -
EFAU085_RS15825 (EFAU085_p3003) 1880..2839 - 960 WP_002287225.1 IS30 family transposase -
EFAU085_RS15830 (EFAU085_p3004) 3022..3873 + 852 WP_002287227.1 hypothetical protein -
EFAU085_RS15835 (EFAU085_p3005) 3894..4106 + 213 WP_002324171.1 hypothetical protein -
EFAU085_RS15840 (EFAU085_p3007) 4539..5219 + 681 WP_002328852.1 IS6-like element IS1216 family transposase -
EFAU085_RS17670 5273..5416 + 144 Protein_6 chromosome partitioning protein ParA -
EFAU085_RS15845 (EFAU085_p3008) 5508..5723 + 216 WP_001835296.1 peptide-binding protein -
EFAU085_RS15850 (EFAU085_p3009) 5740..6012 + 273 WP_000301765.1 antitoxin Antitoxin
EFAU085_RS15855 (EFAU085_p3010) 6014..6877 + 864 WP_001284311.1 zeta toxin family protein Toxin
EFAU085_RS15860 (EFAU085_p3012) 7140..7877 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EFAU085_RS15865 8002..8085 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
EFAU085_RS15870 (EFAU085_p3013) 8627..9421 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EFAU085_RS17310 9514..9765 - 252 Protein_13 GNAT family N-acetyltransferase -
EFAU085_RS15875 (EFAU085_p3014) 9884..10792 + 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
EFAU085_RS15880 (EFAU085_p3015) 10816..11043 - 228 Protein_15 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 1..31004 31004
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 1880..28310 26430


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T29899 WP_001284311.1 NC_021988:6014-6877 [Enterococcus faecium Aus0085]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T29899 NC_021988:6014-6877 [Enterococcus faecium Aus0085]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT29899 WP_000301765.1 NC_021988:5740-6012 [Enterococcus faecium Aus0085]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT29899 NC_021988:5740-6012 [Enterococcus faecium Aus0085]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References