Detailed information of TA system TA029898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 123547..124066
• Replicon: plasmid p1
• Accession: NC_021987
• Organism: Enterococcus faecium Aus0085

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: EFAU085_RS15395
• Protein ID: WP_000588503.1
• Coordinates: 123809..124066 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: -
• Locus tag: EFAU085_RS15390
• Protein ID: WP_020944803.1
• Coordinates: 123547..123816 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFAU085_RS15335 (EFAU085_p1142)	118747..119646	+	900	WP_002303312.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
EFAU085_RS15340 (EFAU085_p1143)	119649..120134	+	486	WP_002303313.1	single-stranded DNA-binding protein	-
EFAU085_RS15345 (EFAU085_p1145)	120262..120438	-	177	WP_230374965.1	putative holin-like toxin	-
EFAU085_RS15350 (EFAU085_p1146)	120552..120812	+	261	WP_002295623.1	hypothetical protein	-
EFAU085_RS15355 (EFAU085_p1147)	121249..121455	+	207	WP_002347218.1	hypothetical protein	-
EFAU085_RS15360 (EFAU085_p1148)	121455..121706	+	252	WP_002296238.1	hypothetical protein	-
EFAU085_RS15365 (EFAU085_p1149)	121721..122158	+	438	WP_002300851.1	hypothetical protein	-
EFAU085_RS17290	122151..122857	+	707	Protein_129	hypothetical protein	-
EFAU085_RS18230 (EFAU085_p1152)	123022..123221	+	200	Protein_130	hypothetical protein	-
EFAU085_RS15385 (EFAU085_p1153)	123237..123416	+	180	WP_001120991.1	hypothetical protein	-
EFAU085_RS15390 (EFAU085_p1154)	123547..123816	+	270	WP_020944803.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EFAU085_RS15395 (EFAU085_p1155)	123809..124066	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
EFAU085_RS15400 (EFAU085_p1157)	124700..125524	+	825	WP_001809248.1	hypothetical protein	-
EFAU085_RS15405 (EFAU085_p1158)	125689..126303	-	615	WP_001261742.1	recombinase family protein	-
EFAU085_RS15410 (EFAU085_p1159)	126541..126963	+	423	WP_002350734.1	hypothetical protein	-
EFAU085_RS18235 (EFAU085_p1160)	126985..127314	+	330	WP_239015693.1	hypothetical protein	-
EFAU085_RS15420 (EFAU085_p1161)	127386..127970	+	585	WP_002299575.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..130716	130716
	-	flank	IS/Tn	-	-	127386..127970	584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T29898 WP_000588503.1 NC_021987:123809-124066 [Enterococcus faecium Aus0085]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T29898 NC_021987:123809-124066 [Enterococcus faecium Aus0085]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT29898 WP_020944803.1 NC_021987:123547-123816 [Enterococcus faecium Aus0085]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT29898 NC_021987:123547-123816 [Enterococcus faecium Aus0085]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8