Detailed information of TA system TA029889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1039541..1040331
• Replicon: plasmid pRetMIM1f
• Accession: NC_021911
• Organism: Rhizobium etli bv. mimosae str. Mim1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: S5S9T1
• Locus tag: REMIM1_RS34225
• Protein ID: WP_020919957.1
• Coordinates: 1039541..1040038 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: S5SHB4
• Locus tag: REMIM1_RS34230
• Protein ID: WP_020919958.1
• Coordinates: 1040035..1040331 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
REMIM1_RS34205	1034637..1034945	+	309	WP_020919953.1	hypothetical protein	-
REMIM1_RS34210	1034942..1035559	+	618	WP_020919954.1	exopolysaccharide biosynthesis protein	-
REMIM1_RS34215	1035717..1037339	-	1623	WP_020919955.1	EAL domain-containing protein	-
REMIM1_RS34220	1037714..1038652	-	939	WP_020919956.1	SGNH family esterase	-
REMIM1_RS34225	1039541..1040038	-	498	WP_020919957.1	GNAT family N-acetyltransferase	Toxin
REMIM1_RS34230	1040035..1040331	-	297	WP_020919958.1	DUF1778 domain-containing protein	Antitoxin
REMIM1_RS34240	1040879..1041427	-	549	WP_011428446.1	DUF1003 domain-containing protein	-
REMIM1_RS34245	1041623..1042528	-	906	WP_020919960.1	NAD(P)/FAD-dependent oxidoreductase	-
REMIM1_RS34255	1043055..1043315	-	261	WP_041679349.1	DUF982 domain-containing protein	-
REMIM1_RS34260	1043717..1044184	-	468	WP_020919963.1	DUF2269 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1082659	1082659

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (36-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-97)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 17831.30 Da
• Isoelectric Point: 7.3587

>T29889 WP_020919957.1 NC_021911:c1040038-1039541 [Rhizobium etli bv. mimosae str. Mim1]
VTLSAPAPLAEHHELAEFNSGVGELDDWLRRRARSNQTSGASRTFVVCEESRVIAYYALASGAVRQPQAPGRFRRNMPDP
IPVAVLGRLAIDQSYQGRGIGRALVRDAGLRLLNAAEVLGIRGMLVHAISDDARAFYEAVGFLSSPSDPMVLMVGLHDLN
NALNP

Nucleotide

• Length: 498 bp

>T29889 NC_021911:c1040038-1039541 [Rhizobium etli bv. mimosae str. Mim1]
GTGACCCTCAGCGCACCGGCACCCCTGGCCGAACATCATGAGCTGGCCGAGTTCAATTCCGGAGTCGGGGAACTTGACGA
CTGGCTGCGACGTCGCGCCCGTTCTAATCAGACAAGCGGCGCATCGCGGACCTTCGTCGTGTGCGAGGAGAGCCGCGTCA
TCGCCTATTACGCGCTCGCTTCCGGCGCCGTCAGACAGCCGCAAGCACCTGGCCGTTTTCGGCGCAATATGCCTGACCCA
ATTCCGGTCGCTGTTCTCGGCCGCCTCGCCATTGATCAATCCTACCAAGGGCGCGGTATCGGCAGGGCATTGGTGCGCGA
CGCGGGCCTGCGCCTGCTCAATGCCGCTGAAGTGCTTGGCATTCGAGGTATGCTCGTTCATGCGATTTCAGATGACGCAC
GAGCCTTCTACGAGGCCGTCGGCTTCCTGTCTTCTCCCTCAGACCCCATGGTGCTGATGGTCGGATTGCATGACCTCAAT
AACGCGCTGAACCCTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11093.58 Da
• Isoelectric Point: 5.8769

>AT29889 WP_020919958.1 NC_021911:c1040331-1040035 [Rhizobium etli bv. mimosae str. Mim1]
MPTSHTSKSSDASVPLNMRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTIFTVSPEIYAEYLTRLDAPTQ
PNERLARTMSTKAPWDEA

Nucleotide

• Length: 297 bp

>AT29889 NC_021911:c1040331-1040035 [Rhizobium etli bv. mimosae str. Mim1]
ATGCCGACCTCACACACGAGCAAGAGCTCGGACGCAAGCGTCCCGCTAAATATGCGCATCAAGCCTGCAACCCGCAATCT
GATCGATCGAGCCGCTGAGTTGCTTGGTAAGACGCGCACGGATTTCATGTTGGAAGCTTCCGAGCGCCGAGCCGAGGAAG
TGCTGCTCGACCGCACTATCTTCACGGTAAGCCCGGAAATCTATGCGGAATACCTTACTCGGCTCGATGCACCCACGCAG
CCCAATGAGCGTCTAGCACGCACCATGTCGACCAAGGCACCGTGGGATGAGGCGTGA

Structures

Toxin

• AlphaFold DB: S5S9T1

Antitoxin

• AlphaFold DB: S5SHB4