Detailed information of TA system TA029887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 204185..204792
• Replicon: plasmid pRetMIM1f
• Accession: NC_021911
• Organism: Rhizobium etli bv. mimosae str. Mim1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S5SES5
• Locus tag: REMIM1_RS30385
• Protein ID: WP_020919230.1
• Coordinates: 204538..204792 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: S5S7D6
• Locus tag: REMIM1_RS30380
• Protein ID: WP_020919229.1
• Coordinates: 204185..204541 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
REMIM1_RS30355	199263..199475	-	213	WP_020919226.1	DUF2283 domain-containing protein	-
REMIM1_RS30360	200119..201057	+	939	Protein_186	ISNCY family transposase	-
REMIM1_RS36260	201315..201665	+	351	Protein_187	IS30 family transposase	-
REMIM1_RS36885	201755..202387	-	633	WP_020919227.1	DUF433 domain-containing protein	-
REMIM1_RS30375	202400..203606	-	1207	Protein_189	putative DNA binding domain-containing protein	-
REMIM1_RS30380	204185..204541	-	357	WP_020919229.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
REMIM1_RS30385	204538..204792	-	255	WP_020919230.1	type II toxin-antitoxin system HicA family toxin	Toxin
REMIM1_RS36265	205077..205544	+	468	WP_080655890.1	IS5 family transposase	-
REMIM1_RS30395	205609..206334	-	726	WP_020919231.1	response regulator	-
REMIM1_RS30400	206467..206799	-	333	WP_020919232.1	hypothetical protein	-
REMIM1_RS30405	206890..207570	-	681	WP_041679287.1	NnrU family protein	-
REMIM1_RS30410	207589..208284	-	696	WP_041679372.1	Crp/Fnr family transcriptional regulator	-
REMIM1_RS30415	208287..209240	-	954	WP_020919235.1	formylglycine-generating enzyme family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1082659	1082659
	-	flank	IS/Tn	-	-	200113..201057	944

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9345.71 Da
• Isoelectric Point: 8.6586

>T29887 WP_020919230.1 NC_021911:c204792-204538 [Rhizobium etli bv. mimosae str. Mim1]
MNSKQRKTLAAVFKDPVSGTIEWAAIENLLVAAGADLIEGNGSRVKFEKDEIIASFHRPHPAKEAKRYQVRDAHEFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T29887 NC_021911:c204792-204538 [Rhizobium etli bv. mimosae str. Mim1]
ATGAACAGCAAGCAAAGAAAGACTCTCGCGGCGGTCTTCAAGGACCCAGTCTCGGGAACCATTGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCCGACCTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGAGATCATTG
CGAGCTTCCATCGACCACATCCGGCCAAGGAAGCGAAGCGTTATCAGGTGCGCGATGCTCACGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13227.94 Da
• Isoelectric Point: 5.5750

>AT29887 WP_020919229.1 NC_021911:c204541-204185 [Rhizobium etli bv. mimosae str. Mim1]
MNIMTYNGYHARIAFDAEDDLFFGRIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAANHFAEARLPA

Nucleotide

• Length: 357 bp

>AT29887 NC_021911:c204541-204185 [Rhizobium etli bv. mimosae str. Mim1]
ATGAACATCATGACCTACAACGGATATCACGCTCGCATCGCATTCGACGCTGAGGACGATCTGTTTTTCGGCAGGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCAGTCGATGACT
ACCTGGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTGAACCAGTGGGCAGAAGAGGTGCTGGAAGAGGC
CGCCAACCATTTTGCCGAGGCGCGCTTGCCGGCGTAG

Structures

Toxin

• AlphaFold DB: S5SES5

Antitoxin

• AlphaFold DB: S5S7D6