Detailed information of TA system TA029886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 196792..197459
• Replicon: plasmid pRetMIM1f
• Accession: NC_021911
• Organism: Rhizobium etli bv. mimosae str. Mim1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: S5S702
• Locus tag: REMIM1_RS30335
• Protein ID: WP_020919222.1
• Coordinates: 196792..197235 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: REMIM1_RS30340
• Protein ID: WP_155249506.1
• Coordinates: 197232..197459 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
REMIM1_RS36250	192293..192570	-	278	Protein_176	LysR family transcriptional regulator	-
REMIM1_RS30310	193001..194176	-	1176	WP_020919216.1	alpha/beta fold hydrolase	-
REMIM1_RS30320	194565..195470	-	906	WP_020919218.1	SDR family oxidoreductase	-
REMIM1_RS30325	195547..195975	+	429	WP_020919219.1	helix-turn-helix transcriptional regulator	-
REMIM1_RS30330	196010..196450	+	441	WP_155249505.1	amidohydrolase family protein	-
REMIM1_RS30335	196792..197235	-	444	WP_020919222.1	PIN domain-containing protein	Toxin
REMIM1_RS30340	197232..197459	-	228	WP_155249506.1	CopG family transcriptional regulator	Antitoxin
REMIM1_RS30345	197941..198159	-	219	WP_020919224.1	helix-turn-helix domain-containing protein	-
REMIM1_RS30350	198249..199138	+	890	Protein_184	LysR family transcriptional regulator	-
REMIM1_RS30355	199263..199475	-	213	WP_020919226.1	DUF2283 domain-containing protein	-
REMIM1_RS30360	200119..201057	+	939	Protein_186	ISNCY family transposase	-
REMIM1_RS36260	201315..201665	+	351	Protein_187	IS30 family transposase	-
REMIM1_RS36885	201755..202387	-	633	WP_020919227.1	DUF433 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1082659	1082659
	-	flank	IS/Tn	-	-	200113..201057	944

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15524.67 Da
• Isoelectric Point: 6.6009

>T29886 WP_020919222.1 NC_021911:c197235-196792 [Rhizobium etli bv. mimosae str. Mim1]
VTFLLDVHVLIALIDPGHVAHDDAHEWFAATGQAAWATCPITENGVIRIVGNPKYPNSPGSPSLVMEIVRKLRSLPGHSF
WPDDVSLVGSGDIDPTRILTPGQVTDTYLLALAKAHGGQLATFDRKLSAAAVTRGNSALHLITASGS

Nucleotide

• Length: 444 bp

>T29886 NC_021911:c197235-196792 [Rhizobium etli bv. mimosae str. Mim1]
GTGACCTTCCTGCTCGACGTCCATGTGCTGATTGCCTTGATCGATCCGGGTCATGTAGCCCATGATGATGCGCACGAGTG
GTTTGCGGCGACGGGTCAGGCCGCTTGGGCTACCTGCCCCATTACCGAGAATGGTGTCATCCGGATTGTCGGCAACCCCA
AATATCCCAACTCTCCCGGCTCACCGTCACTCGTGATGGAGATCGTCCGCAAATTGCGGTCGCTCCCCGGTCACAGTTTT
TGGCCGGACGATGTGAGCCTTGTCGGTTCAGGTGATATCGATCCTACGAGAATCCTAACACCGGGCCAGGTGACTGACAC
CTATCTGCTTGCACTAGCTAAAGCGCATGGGGGCCAGCTGGCGACATTCGATCGCAAGCTGTCAGCAGCAGCGGTTACAA
GGGGCAATTCTGCCTTGCACCTGATCACCGCGAGTGGATCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8170.40 Da
• Isoelectric Point: 7.0120

>AT29886 WP_155249506.1 NC_021911:c197459-197232 [Rhizobium etli bv. mimosae str. Mim1]
MRTTLAIDDDVLIAAKAMATQQHRSVGEVISELARRSLRRPPSSGERNGIPLLSARPDAPLVTLEIVNALRDELP

Nucleotide

• Length: 228 bp

>AT29886 NC_021911:c197459-197232 [Rhizobium etli bv. mimosae str. Mim1]
ATGAGAACGACCTTGGCAATTGACGACGATGTCCTGATCGCCGCAAAAGCGATGGCAACCCAGCAGCACCGGAGCGTCGG
CGAAGTCATTTCTGAGCTGGCGAGACGTTCTCTGCGCCGACCGCCCAGCAGCGGCGAGCGCAATGGCATTCCTCTCCTGT
CAGCTCGGCCGGATGCGCCGCTAGTGACGCTCGAAATCGTGAACGCCTTGCGTGACGAACTGCCGTGA

Structures

Toxin

• AlphaFold DB: S5S702