Detailed information of TA system TA029885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 168618..169198
• Replicon: plasmid pRetMIM1f
• Accession: NC_021911
• Organism: Rhizobium etli bv. mimosae str. Mim1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: S5SW98
• Locus tag: REMIM1_RS30180
• Protein ID: WP_020919193.1
• Coordinates: 168618..169001 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: REMIM1_RS30185
• Protein ID: WP_041679282.1
• Coordinates: 168998..169198 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
REMIM1_RS30175	167961..168455	+	495	WP_155249485.1	hypothetical protein	-
REMIM1_RS30180	168618..169001	-	384	WP_020919193.1	type II toxin-antitoxin system VapC family toxin	Toxin
REMIM1_RS30185	168998..169198	-	201	WP_041679282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
REMIM1_RS30190	169547..169990	+	444	WP_020919194.1	acetyl-CoA carboxylase biotin carboxyl carrier protein subunit	-
REMIM1_RS30195	169990..171384	+	1395	WP_020919195.1	acetyl-CoA carboxylase biotin carboxylase subunit	-
REMIM1_RS30200	171381..171797	+	417	WP_020919196.1	hypothetical protein	-
REMIM1_RS30205	171794..172501	+	708	WP_020919197.1	5-oxoprolinase subunit PxpB	-
REMIM1_RS30210	172498..173487	+	990	WP_020919198.1	biotin-dependent carboxyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1082659	1082659

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14273.45 Da
• Isoelectric Point: 6.6036

>T29885 WP_020919193.1 NC_021911:c169001-168618 [Rhizobium etli bv. mimosae str. Mim1]
VILVDTSIWIDHFRHRDAELSKIIEEDRLLCHPFVIGELALGSLRDRDAVMAFLAAQREAFIATHDEVMTMIDRHLIFSM
GIGYTDAHLLASTLLDRRSTLWTRDKRLAAAAQKAGASLHVPVNTPN

Nucleotide

• Length: 384 bp

>T29885 NC_021911:c169001-168618 [Rhizobium etli bv. mimosae str. Mim1]
GTGATACTGGTTGACACCTCAATTTGGATTGATCATTTCCGCCATAGAGACGCCGAATTAAGCAAAATTATCGAAGAAGA
TCGCCTACTCTGCCACCCTTTCGTAATCGGCGAGTTGGCGCTTGGCAGTCTTCGGGATCGTGATGCCGTGATGGCTTTCC
TAGCGGCGCAGCGGGAAGCATTTATCGCAACGCACGATGAGGTCATGACGATGATCGATCGACATTTGATCTTCAGCATG
GGGATTGGCTATACGGATGCGCACCTGCTGGCTTCGACGCTGCTCGACCGGCGGTCGACTTTGTGGACAAGAGACAAGCG
CCTGGCAGCAGCGGCTCAAAAGGCAGGCGCGTCACTTCATGTACCCGTGAACACACCCAACTAG

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7190.32 Da
• Isoelectric Point: 11.4483

>AT29885 WP_041679282.1 NC_021911:c169198-168998 [Rhizobium etli bv. mimosae str. Mim1]
MRSTINLDDNLIKRARSLTGTKETAALVRQALETLVRVESGKRLVALGGTMPDAEAAPRRRSEAVK

Nucleotide

• Length: 201 bp

>AT29885 NC_021911:c169198-168998 [Rhizobium etli bv. mimosae str. Mim1]
ATGCGATCTACCATTAATCTCGACGACAACCTCATTAAAAGAGCGAGGTCTCTAACCGGCACGAAGGAAACGGCTGCGCT
TGTCCGTCAAGCATTGGAGACACTCGTACGGGTCGAGTCCGGAAAGCGCCTCGTTGCACTTGGGGGCACCATGCCCGACG
CAGAGGCAGCCCCTCGTCGGCGGAGTGAAGCGGTCAAGTGA

Structures

Toxin

• AlphaFold DB: S5SW98