Detailed information of TA system TA029871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2635578..2636293
• Replicon: chromosome
• Accession: NC_021883
• Organism: Mannheimia haemolytica USMARC 2286

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: N220_RS13320
• Protein ID: WP_006250491.1
• Coordinates: 2635865..2636293 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A248ZW87
• Locus tag: N220_RS13315
• Protein ID: WP_006248272.1
• Coordinates: 2635578..2635865 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N220_RS13300	2631130..2631330	-	201	WP_020910234.1	PT domain-containing protein	-
N220_RS13305	2631438..2633153	-	1716	WP_006248268.1	proline--tRNA ligase	-
N220_RS14180	2633317..2633478	-	162	WP_015483997.1	hypothetical protein	-
N220_RS13310	2633531..2635414	-	1884	WP_006248270.1	molecular chaperone HtpG	-
N220_RS13315	2635578..2635865	+	288	WP_006248272.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
N220_RS13320	2635865..2636293	+	429	WP_006250491.1	type II toxin-antitoxin system VapC family toxin	Toxin
N220_RS13325	2636297..2638378	+	2082	WP_006248274.1	ATP-dependent DNA helicase RecG	-
N220_RS13330	2638400..2639334	-	935	Protein_2592	nucleoside hydrolase	-
N220_RS13335	2639387..2641039	-	1653	WP_006248275.1	phospho-sugar mutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16640.06 Da
• Isoelectric Point: 8.9269

>T29871 WP_006250491.1 NC_021883:2635865-2636293 [Mannheimia haemolytica USMARC_2286]
MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCITLSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQT
KAFVVNNDIALLASEYHIPNKMDLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA

Nucleotide

• Length: 429 bp

>T29871 NC_021883:2635865-2636293 [Mannheimia haemolytica USMARC_2286]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11085.39 Da
• Isoelectric Point: 4.3648

>AT29871 WP_006248272.1 NC_021883:2635578-2635865 [Mannheimia haemolytica USMARC_2286]
MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDYQQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVE
IRKSNKPRPVDFGDE

Nucleotide

• Length: 288 bp

>AT29871 NC_021883:2635578-2635865 [Mannheimia haemolytica USMARC_2286]
ATGGCAATAACCATTACTTCCAGAGAATTTAATCAAAATGCCAGCAAAGTGTTAAAAATGGCAGAAAAAGAGCCGGTTTT
TATTACCAAATGGGGCAAAATCGTCAATGTGGTGTCTAGTTATATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCT
TTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGATGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAA
ATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A248ZW87