29870

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2407761..2408350 Replicon chromosome
Accession NC_021883
Organism Mannheimia haemolytica USMARC 2286

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag N220_RS12135 Protein ID WP_006248516.1
Coordinates 2408018..2408350 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag N220_RS12130 Protein ID WP_006248517.1
Coordinates 2407761..2408021 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N220_RS12105 2402940..2404085 - 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -
N220_RS12110 2404697..2405341 + 645 WP_006248521.1 RNA pyrophosphohydrolase -
N220_RS12115 2405343..2406131 + 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
N220_RS12120 2406139..2406936 + 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
N220_RS12125 2407023..2407691 + 669 WP_006248518.1 phosphoglycolate phosphatase -
N220_RS12130 2407761..2408021 + 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
N220_RS12135 2408018..2408350 + 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
N220_RS12140 2408477..2409508 + 1032 WP_006248514.1 tryptophan--tRNA ligase -
N220_RS12145 2409572..2410327 - 756 WP_006248513.1 M48 family metallopeptidase -
N220_RS12150 2410383..2410910 - 528 WP_006248512.1 inorganic diphosphatase -
N220_RS12170 2411490..2412524 + 1035 WP_006248509.1 endolytic transglycosylase MltG -
N220_RS12175 2412535..2413155 + 621 WP_006248508.1 dTMP kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T29870 WP_006248516.1 NC_021883:2408018-2408350 [Mannheimia haemolytica USMARC_2286]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T29870 NC_021883:2408018-2408350 [Mannheimia haemolytica USMARC_2286]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT29870 WP_006248517.1 NC_021883:2407761-2408021 [Mannheimia haemolytica USMARC_2286]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT29870 NC_021883:2407761-2408021 [Mannheimia haemolytica USMARC_2286]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References