Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1414477..1415125 Replicon chromosome
Accession NC_021883
Organism Mannheimia haemolytica USMARC 2286

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag N220_RS06970 Protein ID WP_006251221.1
Coordinates 1414477..1414653 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag N220_RS06975 Protein ID WP_006251222.1
Coordinates 1414709..1415125 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N220_RS06945 1409795..1412290 - 2496 WP_006251216.1 phage tail length tape measure family protein -
N220_RS06955 1412835..1413293 - 459 WP_006251218.1 hypothetical protein -
N220_RS06960 1413462..1413695 + 234 WP_020824316.1 hypothetical protein -
N220_RS06965 1413710..1414381 - 672 WP_006251220.1 hypothetical protein -
N220_RS06970 1414477..1414653 + 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
N220_RS06975 1414709..1415125 + 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
N220_RS06980 1415165..1415647 - 483 WP_006251223.1 phage tail protein -
N220_RS06985 1415651..1416031 - 381 WP_006251224.1 hypothetical protein -
N220_RS06990 1416028..1416396 - 369 WP_006251225.1 hypothetical protein -
N220_RS06995 1416398..1416742 - 345 WP_006251226.1 hypothetical protein -
N220_RS07000 1416742..1417119 - 378 WP_006251227.1 hypothetical protein -
N220_RS07005 1417122..1417391 - 270 WP_020849873.1 hypothetical protein -
N220_RS07010 1417403..1418392 - 990 WP_006251229.1 encapsulin -
N220_RS07015 1418407..1418841 - 435 WP_006251230.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1390213..1477849 87636


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T29867 WP_006251221.1 NC_021883:1414477-1414653 [Mannheimia haemolytica USMARC_2286]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T29867 NC_021883:1414477-1414653 [Mannheimia haemolytica USMARC_2286]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT29867 WP_006251222.1 NC_021883:1414709-1415125 [Mannheimia haemolytica USMARC_2286]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT29867 NC_021883:1414709-1415125 [Mannheimia haemolytica USMARC_2286]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References