Detailed information of TA system TA029857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 106491..107028
• Replicon: chromosome
• Accession: NC_021877
• Organism: Burkholderia pseudomallei MSHR305

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BDL_RS00465
• Protein ID: WP_009923450.1
• Coordinates: 106491..106760 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BDL_RS00470
• Protein ID: WP_004525420.1
• Coordinates: 106744..107028 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BDL_RS41905	102468..102896	-	429	Protein_78	DDE-type integrase/transposase/recombinase	-
BDL_RS40975	102894..103667	-	774	Protein_79	prohibitin family protein	-
BDL_RS00425	103664..103813	-	150	WP_004542626.1	bacteriophage protein Gp44	-
BDL_RS00430	104069..104650	+	582	WP_004542548.1	hypothetical protein	-
BDL_RS40980	104881..105099	-	219	WP_004552392.1	hypothetical protein	-
BDL_RS00440	105147..105368	-	222	WP_004525418.1	hypothetical protein	-
BDL_RS00455	105645..105920	-	276	WP_004552388.1	bacteriophage protein Gp49	-
BDL_RS00465	106491..106760	+	270	WP_009923450.1	BrnT family toxin	Toxin
BDL_RS00470	106744..107028	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BDL_RS00480	107675..108058	+	384	Protein_87	DDE-type integrase/transposase/recombinase	-
BDL_RS32420	108021..108449	+	429	Protein_88	IS110 family transposase	-
BDL_RS00485	108676..108792	-	117	Protein_89	IS3 family transposase	-
BDL_RS00490	109039..110499	+	1461	WP_020850157.1	metallophosphoesterase	-
BDL_RS00495	110658..111920	+	1263	WP_020850375.1	cytochrome c family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	99783..110499	10716

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10156.52 Da
• Isoelectric Point: 7.1633

>T29857 WP_009923450.1 NC_021877:106491-106760 [Burkholderia pseudomallei MSHR305]
MDITFDPTKNKTNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T29857 NC_021877:106491-106760 [Burkholderia pseudomallei MSHR305]
ATGGACATCACCTTTGACCCGACCAAGAACAAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT29857 WP_004525420.1 NC_021877:106744-107028 [Burkholderia pseudomallei MSHR305]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT29857 NC_021877:106744-107028 [Burkholderia pseudomallei MSHR305]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8