Detailed information of TA system TA029855

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 68640..69443
• Replicon: plasmid RM1
• Accession: NC_021871
• Organism: Salmonella bongori N268-08

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: S5N4E8
• Locus tag: A464_RS22925
• Protein ID: WP_020842550.1
• Coordinates: 68640..69170 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: S5N4Z2
• Locus tag: A464_RS22930
• Protein ID: WP_020842551.1
• Coordinates: 69174..69443 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A464_RS22910	64083..66677	+	2595	WP_020842542.1	autotransporter outer membrane beta-barrel domain-containing protein	-
A464_RS22915	66842..67378	+	537	WP_020842543.1	Vi polysaccharide biosynthesis protein tviA	-
A464_RS24505	67436..67725	-	290	Protein_79	transposase	-
A464_RS25370	67822..68097	-	276	Protein_80	IS3 family transposase	-
A464_RS24515	68093..68293	+	201	Protein_81	transposase	-
A464_RS22925	68640..69170	-	531	WP_020842550.1	GNAT family N-acetyltransferase	Toxin
A464_RS22930	69174..69443	-	270	WP_020842551.1	DUF1778 domain-containing protein	Antitoxin
A464_RS22940	70293..71282	+	990	WP_077909641.1	RepB family plasmid replication initiator protein	-
A464_RS22945	71529..72314	-	786	WP_049823145.1	site-specific integrase	-
A464_RS25380	72428..72553	-	126	WP_138993539.1	transcriptional regulator	-
A464_RS22950	72662..72958	-	297	WP_020842555.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A464_RS22955	72945..73211	-	267	WP_020842556.1	type II toxin-antitoxin system ParD family antitoxin	-
A464_RS22960	73370..73713	+	344	Protein_89	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..89986	89986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20407.43 Da
• Isoelectric Point: 7.1048

>T29855 WP_020842550.1 NC_021871:c69170-68640 [Salmonella bongori N268-08]
MDSLRIEIFSEEVEYELSNFDCGEEHLNTFLTNHLKRQHKSKILRGYVLVTREDKPRVMGFYTLSGSCFERILLLSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVIHAMKVVYQASQAVGIHGMFVEALNDNAQKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVEDK

Nucleotide

• Length: 531 bp

>T29855 NC_021871:c69170-68640 [Salmonella bongori N268-08]
ATGGATAGTTTAAGGATTGAGATTTTTTCTGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGCATCT
GAATACTTTTCTTACTAACCATCTGAAACGTCAGCACAAAAGTAAAATTTTACGCGGTTATGTGCTCGTGACGAGAGAGG
ATAAACCGCGTGTTATGGGTTTCTACACGCTGTCTGGCAGTTGCTTTGAGAGAATACTTCTACTCAGCAAAACTCAACAA
AAGCGCGTGCCCTATAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTATCCATGCTATGAAAGTCGTGTATCAGGCTTCGCAAGCAGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCCAAAAATTCTACCTTCGTCTGGGTTTTATACAGCTAAAGGAAGAAAACTGTAACTCGCTC
TTCTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAGAAGACAAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10334.86 Da
• Isoelectric Point: 8.6583

>AT29855 WP_020842551.1 NC_021871:c69443-69174 [Salmonella bongori N268-08]
MSTLKKQRIDLRLNEYDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMEAITNPPAPNDRLKR
AAKRLQELK

Nucleotide

• Length: 270 bp

>AT29855 NC_021871:c69443-69174 [Salmonella bongori N268-08]
ATGTCAACACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAATACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACTGCCTCAGAACGTGCTGCAGAAGTTATTGATCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCCTGGAACCTGGTTATGGAAGCAATCACTAATCCGCCAGCACCGAATGACCGGCTGAAACGA
GCCGCTAAACGTCTTCAGGAACTGAAATAA

Structures

Toxin

• AlphaFold DB: S5N4E8

Antitoxin

• AlphaFold DB: S5N4Z2