Detailed information of TA system TA029854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 61630..62285
• Replicon: plasmid RM1
• Accession: NC_021871
• Organism: Salmonella bongori N268-08

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: A464_RS22895
• Protein ID: WP_042917490.1
• Coordinates: 61857..62285 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: S5N4X7
• Locus tag: A464_RS22890
• Protein ID: WP_020842536.1
• Coordinates: 61630..61860 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A464_RS22865	56676..57756	-	1081	Protein_67	LD-carboxypeptidase	-
A464_RS22870	58315..59256	+	942	WP_020842533.1	Rpn family recombination-promoting nuclease/putative transposase	-
A464_RS22875	59407..60189	-	783	WP_020842534.1	site-specific integrase	-
A464_RS24895	60227..60361	-	135	Protein_70	cytoplasmic protein	-
A464_RS22880	60434..60739	-	306	WP_020842535.1	type II toxin-antitoxin system toxin CcdB	-
A464_RS22885	60741..60959	-	219	WP_000813642.1	type II toxin-antitoxin system antitoxin CcdA	-
A464_RS22890	61630..61860	+	231	WP_020842536.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A464_RS22895	61857..62285	+	429	WP_042917490.1	type II toxin-antitoxin system VapC family toxin	Toxin
A464_RS25360	62512..62757	-	246	WP_138993537.1	hypothetical protein	-
A464_RS22905	63002..63781	-	780	WP_020842541.1	winged helix-turn-helix domain-containing protein	-
A464_RS22910	64083..66677	+	2595	WP_020842542.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..89986	89986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-133)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15650.17 Da
• Isoelectric Point: 8.7546

>T29854 WP_042917490.1 NC_021871:61857-62285 [Salmonella bongori N268-08]
MKQPRTKTYMLDTCICSFIMREQPDAVLKRLEQAVLRGHRIVISAITYSEIRFGATGPKASPRHVQLVEDFCARLDAILP
WNRAAIDATTEIKVALRLTGTPLGPNDTAIAGHAISAGAVLVTNNVREFARVPGLEPEDWVK

Nucleotide

• Length: 429 bp

>T29854 NC_021871:61857-62285 [Salmonella bongori N268-08]
ATGAAACAGCCACGCACAAAGACGTATATGCTCGATACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGACGCGGT
GCTGAAGCGCCTGGAGCAGGCGGTGCTGCGCGGCCACCGTATCGTGATCTCGGCCATTACCTACTCGGAAATACGTTTCG
GCGCCACCGGCCCGAAGGCTTCGCCACGCCACGTGCAGCTGGTTGAGGACTTCTGCGCGCGCCTCGATGCCATTCTGCCC
TGGAACCGGGCTGCGATCGACGCCACCACGGAGATTAAGGTGGCACTACGCCTCACCGGGACGCCGCTCGGCCCGAACGA
CACAGCAATTGCCGGACACGCCATCTCCGCCGGCGCCGTGCTGGTGACGAATAATGTGAGAGAGTTTGCGCGGGTGCCGG
GGTTAGAGCCTGAAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8841.99 Da
• Isoelectric Point: 4.3894

>AT29854 WP_020842536.1 NC_021871:61630-61860 [Salmonella bongori N268-08]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIIREGDCIILRPVRPTWSSFAQLEKTDPDFMAEREEVVSDEGRFEI

Nucleotide

• Length: 231 bp

>AT29854 NC_021871:61630-61860 [Salmonella bongori N268-08]
ATGAGAACCGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCATCCGGGAAGGGGACTGCATTATCCTGCGCCCCGTCCGGCCGACCTGGAGCTCGTTCGCGCAGCTGG
AAAAAACCGACCCGGACTTTATGGCGGAGCGTGAGGAGGTTGTCAGTGACGAAGGACGCTTTGAAATATGA

Structures

Antitoxin

• AlphaFold DB: S5N4X7