Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 51351..52974 Replicon plasmid unnamed
Accession NC_021819
Organism Salmonella enterica subsp. enterica serovar Cubana str. CFSAN002050

Toxin (Protein)


Gene name hipA Uniprot ID A0A7D6U035
Locus tag CFSAN002050_RS25305 Protein ID WP_020833804.1
Coordinates 51351..52676 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5V9NJV0
Locus tag CFSAN002050_RS25310 Protein ID WP_024143021.1
Coordinates 52681..52974 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFSAN002050_RS25290 47756..48658 - 903 WP_020833802.1 WYL domain-containing protein -
CFSAN002050_RS25295 48822..50420 - 1599 WP_020833803.1 hypothetical protein -
CFSAN002050_RS27340 50420..50914 - 495 WP_071685143.1 hypothetical protein -
CFSAN002050_RS25300 51084..51290 - 207 WP_000134171.1 hypothetical protein -
CFSAN002050_RS25305 51351..52676 - 1326 WP_020833804.1 type II toxin-antitoxin system HipA family toxin Toxin
CFSAN002050_RS25310 52681..52974 - 294 WP_024143021.1 helix-turn-helix transcriptional regulator Antitoxin
CFSAN002050_RS25315 53375..54727 - 1353 WP_049827791.1 hypothetical protein -
CFSAN002050_RS25325 54809..56049 + 1241 Protein_56 IS3 family transposase -
CFSAN002050_RS25330 56169..56456 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE -
CFSAN002050_RS25335 56456..56695 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB -
CFSAN002050_RS25340 56958..57881 - 924 WP_000167917.1 cation diffusion facilitator family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-9 - 1..122863 122863
- flank IS/Tn - - 45580..47100 1520
- inside IScluster/Tn - - 55162..62883 7721


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-70)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50307.23 Da        Isoelectric Point: 6.0252

>T29822 WP_020833804.1 NC_021819:c52676-51351 [Salmonella enterica subsp. enterica serovar Cubana str. CFSAN002050]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLDQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T29822 NC_021819:c52676-51351 [Salmonella enterica subsp. enterica serovar Cubana str. CFSAN002050]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGACCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCTTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10990.39 Da        Isoelectric Point: 9.9492

>AT29822 WP_024143021.1 NC_021819:c52974-52681 [Salmonella enterica subsp. enterica serovar Cubana str. CFSAN002050]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSVLGVKISFSSATTSSERK
QTEEIYKLNSPARQEDW

Download         Length: 294 bp

>AT29822 NC_021819:c52974-52681 [Salmonella enterica subsp. enterica serovar Cubana str. CFSAN002050]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGTGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCGTCCTGGGTGTAAAAATCAGCTTCTCCTCGGCAACCACTTCTTCAGAGAGGAAG
CAGACGGAAGAAATATATAAATTAAATTCACCTGCACGACAGGAGGATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6U035

Antitoxin

Source ID Structure
AlphaFold DB A0A5V9NJV0

References