Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-MazE
Location 2072455..2073105 Replicon chromosome
Accession NC_021743
Organism Mannheimia haemolytica D153

Toxin (Protein)


Gene name vapC Uniprot ID A0A547EDJ6
Locus tag F382_RS10490 Protein ID WP_006249411.1
Coordinates 2072716..2073105 (+) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A547EDM1
Locus tag F382_RS10485 Protein ID WP_006249412.1
Coordinates 2072455..2072715 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F382_RS10470 2068143..2071676 + 3534 WP_006253629.1 transcription-repair coupling factor -
F382_RS10475 2071839..2072126 + 288 WP_006251764.1 BrnT family toxin -
F382_RS10480 2072127..2072339 + 213 WP_006251765.1 BrnA antitoxin family protein -
F382_RS10485 2072455..2072715 + 261 WP_006249412.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
F382_RS10490 2072716..2073105 + 390 WP_006249411.1 type II toxin-antitoxin system VapC family toxin Toxin
F382_RS10495 2073125..2074363 + 1239 WP_006253630.1 peptidase T -
F382_RS10515 2074898..2075683 + 786 WP_006251768.1 hypothetical protein -
F382_RS10520 2075757..2076617 + 861 WP_006250124.1 metal ABC transporter permease -
F382_RS10525 2076610..2077419 + 810 WP_006250123.1 metal ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-119)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14633.91 Da        Isoelectric Point: 7.1041

>T29785 WP_006249411.1 NC_021743:2072716-2073105 [Mannheimia haemolytica D153]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Download         Length: 390 bp

>T29785 NC_021743:2072716-2073105 [Mannheimia haemolytica D153]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGATTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGTGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 10245.54 Da        Isoelectric Point: 4.8951

>AT29785 WP_006249412.1 NC_021743:2072455-2072715 [Mannheimia haemolytica D153]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Download         Length: 261 bp

>AT29785 NC_021743:2072455-2072715 [Mannheimia haemolytica D153]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAAAAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A547EDJ6

Antitoxin

Source ID Structure
AlphaFold DB A0A547EDM1

References