Detailed information of TA system TA029784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 1586917..1587632
• Replicon: chromosome
• Accession: NC_021743
• Organism: Mannheimia haemolytica D153

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F382_RS08055
• Protein ID: WP_006250491.1
• Coordinates: 1586917..1587345 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A248ZW87
• Locus tag: F382_RS08060
• Protein ID: WP_006248272.1
• Coordinates: 1587345..1587632 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F382_RS08040	1582171..1583823	+	1653	WP_006248275.1	phospho-sugar mutase	-
F382_RS08045	1583876..1584810	+	935	Protein_1578	nucleoside hydrolase	-
F382_RS08050	1584832..1586913	-	2082	WP_006248274.1	ATP-dependent DNA helicase RecG	-
F382_RS08055	1586917..1587345	-	429	WP_006250491.1	type II toxin-antitoxin system VapC family toxin	Toxin
F382_RS08060	1587345..1587632	-	288	WP_006248272.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
F382_RS08065	1587796..1589679	+	1884	WP_006248270.1	molecular chaperone HtpG	-
F382_RS14405	1589732..1589893	+	162	WP_015483997.1	hypothetical protein	-
F382_RS08070	1590057..1591772	+	1716	WP_006248268.1	proline--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16640.06 Da
• Isoelectric Point: 8.9269

>T29784 WP_006250491.1 NC_021743:c1587345-1586917 [Mannheimia haemolytica D153]
MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCITLSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQT
KAFVVNNDIALLASEYHIPNKMDLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA

Nucleotide

• Length: 429 bp

>T29784 NC_021743:c1587345-1586917 [Mannheimia haemolytica D153]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11085.39 Da
• Isoelectric Point: 4.3648

>AT29784 WP_006248272.1 NC_021743:c1587632-1587345 [Mannheimia haemolytica D153]
MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDYQQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVE
IRKSNKPRPVDFGDE

Nucleotide

• Length: 288 bp

>AT29784 NC_021743:c1587632-1587345 [Mannheimia haemolytica D153]
ATGGCAATAACCATTACTTCCAGAGAATTTAATCAAAATGCCAGCAAAGTGTTAAAAATGGCAGAAAAAGAGCCGGTTTT
TATTACCAAATGGGGCAAAATCGTCAATGTGGTGTCTAGTTATATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCT
TTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGATGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAA
ATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A248ZW87