Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 472910..473550 Replicon chromosome
Accession NC_021743
Organism Mannheimia haemolytica D153

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag F382_RS02385 Protein ID WP_006250075.1
Coordinates 472910..473092 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NFW9
Locus tag F382_RS02390 Protein ID WP_006250074.1
Coordinates 473131..473550 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F382_RS02345 468457..468750 - 294 WP_006247812.1 hypothetical protein -
F382_RS02350 468894..469283 + 390 WP_015484247.1 type II toxin-antitoxin system RelE/ParE family toxin -
F382_RS02355 469255..469554 + 300 WP_006247810.1 XRE family transcriptional regulator -
F382_RS14320 469563..469721 - 159 WP_006247809.1 hypothetical protein -
F382_RS14325 469731..470798 - 1068 WP_020828775.1 hypothetical protein -
F382_RS02365 470819..471019 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
F382_RS02370 471150..471833 + 684 WP_006248824.1 helix-turn-helix transcriptional regulator -
F382_RS02375 471908..472288 + 381 WP_006248823.1 hypothetical protein -
F382_RS02380 472293..472778 + 486 WP_171814961.1 hypothetical protein -
F382_RS02385 472910..473092 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
F382_RS02390 473131..473550 + 420 WP_006250074.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
F382_RS02395 473886..474530 + 645 WP_006253274.1 tyrosine-type recombinase/integrase -
F382_RS02400 474668..475708 + 1041 WP_015586960.1 IS481 family transposase -
F382_RS02405 475775..477181 - 1407 WP_006253513.1 YdgA family protein -
F382_RS02410 477231..478037 - 807 WP_006248821.1 tryptophan synthase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 465314..477181 11867
- flank IS/Tn - - 474668..475708 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T29779 WP_006250075.1 NC_021743:472910-473092 [Mannheimia haemolytica D153]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T29779 NC_021743:472910-473092 [Mannheimia haemolytica D153]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15938.30 Da        Isoelectric Point: 4.6963

>AT29779 WP_006250074.1 NC_021743:473131-473550 [Mannheimia haemolytica D153]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Download         Length: 420 bp

>AT29779 NC_021743:473131-473550 [Mannheimia haemolytica D153]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NFW9

References