Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-PHD
Location 3780241..3780908 Replicon chromosome
Accession NC_021740
Organism Mycobacterium tuberculosis EAI5

Toxin (Protein)


Gene name vapC Uniprot ID O50411
Locus tag M943_RS17740 Protein ID WP_003417916.1
Coordinates 3780241..3780633 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8U8S7
Locus tag M943_RS17745 Protein ID WP_003912214.1
Coordinates 3780633..3780908 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M943_RS17725 3775611..3777449 - 1839 WP_019830554.1 1-deoxy-D-xylulose-5-phosphate synthase -
M943_RS17730 3777446..3778435 - 990 WP_003417912.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
M943_RS17735 3778435..3779487 - 1053 WP_003900678.1 polyprenyl synthetase family protein -
M943_RS17740 3780241..3780633 - 393 WP_003417916.1 type II toxin-antitoxin system VapC family toxin Toxin
M943_RS17745 3780633..3780908 - 276 WP_003912214.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
M943_RS21630 3781120..3782461 + 1342 Protein_3484 ISNCY family transposase -
M943_RS17760 3782651..3784846 + 2196 WP_010886168.1 PE family protein -
M943_RS17765 3784917..3785789 - 873 WP_003417929.1 3-hydroxyacyl-thioester dehydratase HtdY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3781784..3782461 677


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14316.74 Da        Isoelectric Point: 10.7249

>T29758 WP_003417916.1 NC_021740:c3780633-3780241 [Mycobacterium tuberculosis EAI5]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRHPRVSSALARAEVMRALLDKGESARKAGRRALAHLDLLRVDKRVLDLAG
GLLPFELRTLDAIHLATAQRLGVDLGRLCTYDDRMRDAAKTLGMAVIAPS

Download         Length: 393 bp

>T29758 NC_021740:c3780633-3780241 [Mycobacterium tuberculosis EAI5]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 9966.35 Da        Isoelectric Point: 4.5332

>AT29758 WP_003912214.1 NC_021740:c3780908-3780633 [Mycobacterium tuberculosis EAI5]
MTSVGVRALRQRASELLRRVEAGETIEITDRGRPVALLSPLPQGGPYEQLLASGEIERATLDVVDLPEPLDLDAGVELPS
VTLARLREHER

Download         Length: 276 bp

>AT29758 NC_021740:c3780908-3780633 [Mycobacterium tuberculosis EAI5]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BXS8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8U8S7

References