Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3691351..3692025 Replicon chromosome
Accession NC_021740
Organism Mycobacterium tuberculosis EAI5

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag M943_RS17435 Protein ID WP_003417282.1
Coordinates 3691351..3691779 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag M943_RS17440 Protein ID WP_003417286.1
Coordinates 3691783..3692025 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M943_RS17410 3687173..3687574 - 402 WP_003417264.1 cytidine deaminase -
M943_RS17415 3687715..3688149 + 435 WP_072460198.1 succinate dehydrogenase, cytochrome b556 subunit -
M943_RS17420 3688146..3688580 + 435 WP_003417269.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
M943_RS17425 3688709..3690481 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
M943_RS17430 3690481..3691272 + 792 WP_020824589.1 succinate dehydrogenase iron-sulfur subunit -
M943_RS17435 3691351..3691779 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
M943_RS17440 3691783..3692025 - 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
M943_RS17445 3692147..3692761 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
M943_RS17450 3692758..3693423 - 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
M943_RS17455 3693424..3693957 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
M943_RS17460 3693954..3694043 - 90 WP_009934953.1 pterin-4-alpha-carbinolamine dehydratase moaB3 -
M943_RS17465 3694103..3695815 + 1713 WP_003905037.1 IS110 family transposase -
M943_RS17470 3695748..3696686 - 939 WP_003417413.1 sigma-70 family RNA polymerase sigma factor SigJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T29756 WP_003417282.1 NC_021740:c3691779-3691351 [Mycobacterium tuberculosis EAI5]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T29756 NC_021740:c3691779-3691351 [Mycobacterium tuberculosis EAI5]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT29756 WP_003417286.1 NC_021740:c3692025-3691783 [Mycobacterium tuberculosis EAI5]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT29756 NC_021740:c3692025-3691783 [Mycobacterium tuberculosis EAI5]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References