Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3123050..3123654 Replicon chromosome
Accession NC_021740
Organism Mycobacterium tuberculosis EAI5

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag M943_RS14835 Protein ID WP_003414492.1
Coordinates 3123050..3123442 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag M943_RS14840 Protein ID WP_003414495.1
Coordinates 3123439..3123654 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M943_RS14805 3118200..3118988 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
M943_RS14810 3119322..3119867 - 546 WP_003913758.1 DUF1802 family protein -
M943_RS14815 3120139..3121023 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
M943_RS14820 3121026..3121913 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
M943_RS14825 3122218..3122763 - 546 WP_003899500.1 DUF1802 family protein -
M943_RS14830 3122760..3123029 - 270 WP_003414489.1 DUF2277 family protein -
M943_RS14835 3123050..3123442 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
M943_RS14840 3123439..3123654 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
M943_RS14845 3123701..3124450 + 750 WP_003414497.1 enoyl-CoA hydratase -
M943_RS14850 3124529..3125611 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
M943_RS14855 3125604..3126914 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
M943_RS14860 3126917..3127744 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
M943_RS14865 3127741..3128652 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T29752 WP_003414492.1 NC_021740:c3123442-3123050 [Mycobacterium tuberculosis EAI5]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T29752 NC_021740:c3123442-3123050 [Mycobacterium tuberculosis EAI5]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT29752 WP_003414495.1 NC_021740:c3123654-3123439 [Mycobacterium tuberculosis EAI5]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT29752 NC_021740:c3123654-3123439 [Mycobacterium tuberculosis EAI5]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References