Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3096597..3097167 Replicon chromosome
Accession NC_021740
Organism Mycobacterium tuberculosis EAI5

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag M943_RS14685 Protein ID WP_003414166.1
Coordinates 3096597..3096953 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag M943_RS14690 Protein ID WP_003901465.1
Coordinates 3096937..3097167 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M943_RS14665 3092049..3093737 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
M943_RS14670 3093741..3094067 - 327 WP_003414157.1 hypothetical protein -
M943_RS14675 3094240..3094827 + 588 WP_003914429.1 DUF3558 family protein -
M943_RS14680 3094846..3096495 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
M943_RS14685 3096597..3096953 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
M943_RS14690 3096937..3097167 - 231 WP_003901465.1 antitoxin MazE Antitoxin
M943_RS14695 3097210..3098253 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
M943_RS22045 3098444..3098719 + 276 WP_003911993.1 DUF1778 domain-containing protein -
M943_RS22210 3098895..3099149 - 255 WP_003917684.1 hypothetical protein -
M943_RS14710 3099297..3099701 + 405 WP_003414181.1 hypothetical protein -
M943_RS14715 3099698..3099889 + 192 WP_003414184.1 hypothetical protein -
M943_RS14720 3099953..3101242 + 1290 Protein_2889 transposase family protein -
M943_RS14725 3101476..3101733 + 258 WP_003899489.1 hypothetical protein -
M943_RS14730 3101838..3102149 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T29750 WP_003414166.1 NC_021740:c3096953-3096597 [Mycobacterium tuberculosis EAI5]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T29750 NC_021740:c3096953-3096597 [Mycobacterium tuberculosis EAI5]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT29750 WP_003901465.1 NC_021740:c3097167-3096937 [Mycobacterium tuberculosis EAI5]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT29750 NC_021740:c3097167-3096937 [Mycobacterium tuberculosis EAI5]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References