Detailed information of TA system TA029749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3057012..3057699
• Replicon: chromosome
• Accession: NC_021740
• Organism: Mycobacterium tuberculosis EAI5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: M943_RS14475
• Protein ID: WP_003414064.1
• Coordinates: 3057304..3057699 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: M943_RS14470
• Protein ID: WP_003414061.1
• Coordinates: 3057012..3057278 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M943_RS14445	3052651..3053553	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
M943_RS14450	3053622..3054374	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
M943_RS14455	3054618..3054893	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
M943_RS14460	3054890..3056512	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
M943_RS14465	3056599..3057015	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
M943_RS14470	3057012..3057278	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M943_RS14475	3057304..3057699	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
M943_RS14480	3057696..3057965	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
M943_RS14485	3057975..3059069	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
M943_RS14490	3059066..3059485	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
M943_RS14495	3059559..3060038	-	480	WP_003414073.1	dihydrofolate reductase	-
M943_RS14500	3060109..3060909	-	801	WP_003911953.1	thymidylate synthase	-
M943_RS14505	3061065..3061802	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T29749 WP_003414064.1 NC_021740:c3057699-3057304 [Mycobacterium tuberculosis EAI5]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T29749 NC_021740:c3057699-3057304 [Mycobacterium tuberculosis EAI5]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT29749 WP_003414061.1 NC_021740:c3057278-3057012 [Mycobacterium tuberculosis EAI5]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT29749 NC_021740:c3057278-3057012 [Mycobacterium tuberculosis EAI5]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64