Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3056599..3057278 Replicon chromosome
Accession NC_021740
Organism Mycobacterium tuberculosis EAI5

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag M943_RS14465 Protein ID WP_003414059.1
Coordinates 3056599..3057015 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag M943_RS14470 Protein ID WP_003414061.1
Coordinates 3057012..3057278 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M943_RS14445 3052651..3053553 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
M943_RS14450 3053622..3054374 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
M943_RS14455 3054618..3054893 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
M943_RS14460 3054890..3056512 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
M943_RS14465 3056599..3057015 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
M943_RS14470 3057012..3057278 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M943_RS14475 3057304..3057699 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
M943_RS14480 3057696..3057965 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
M943_RS14485 3057975..3059069 - 1095 WP_003414068.1 restriction endonuclease subunit S -
M943_RS14490 3059066..3059485 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
M943_RS14495 3059559..3060038 - 480 WP_003414073.1 dihydrofolate reductase -
M943_RS14500 3060109..3060909 - 801 WP_003911953.1 thymidylate synthase -
M943_RS14505 3061065..3061802 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T29748 WP_003414059.1 NC_021740:c3057015-3056599 [Mycobacterium tuberculosis EAI5]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T29748 NC_021740:c3057015-3056599 [Mycobacterium tuberculosis EAI5]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCTGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT29748 WP_003414061.1 NC_021740:c3057278-3057012 [Mycobacterium tuberculosis EAI5]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT29748 NC_021740:c3057278-3057012 [Mycobacterium tuberculosis EAI5]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References