Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2856393..2857024 Replicon chromosome
Accession NC_021740
Organism Mycobacterium tuberculosis EAI5

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag M943_RS13390 Protein ID WP_003413174.1
Coordinates 2856647..2857024 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag M943_RS13385 Protein ID WP_003413167.1
Coordinates 2856393..2856650 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M943_RS13355 2852307..2852621 + 315 WP_009937839.1 hypothetical protein -
M943_RS13360 2852917..2854128 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
M943_RS13365 2854255..2854914 + 660 WP_003900846.1 LppA family lipoprotein -
M943_RS13370 2854911..2855573 + 663 WP_020824552.1 hypothetical protein -
M943_RS13375 2855570..2855848 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
M943_RS13380 2855941..2856354 + 414 WP_003413164.1 PIN domain nuclease -
M943_RS13385 2856393..2856650 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
M943_RS13390 2856647..2857024 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
M943_RS13395 2857040..2857414 - 375 WP_003413177.1 hypothetical protein -
M943_RS13400 2857514..2857909 - 396 WP_015631452.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
M943_RS13405 2857906..2858151 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
M943_RS13410 2858562..2858981 - 420 WP_003413190.1 A24 family peptidase -
M943_RS13415 2858993..2859802 - 810 WP_003413193.1 shikimate dehydrogenase -
M943_RS13420 2859799..2861052 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
M943_RS13425 2861045..2861557 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T29744 WP_003413174.1 NC_021740:2856647-2857024 [Mycobacterium tuberculosis EAI5]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T29744 NC_021740:2856647-2857024 [Mycobacterium tuberculosis EAI5]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT29744 WP_003413167.1 NC_021740:2856393-2856650 [Mycobacterium tuberculosis EAI5]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT29744 NC_021740:2856393-2856650 [Mycobacterium tuberculosis EAI5]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References