Detailed information of TA system TA029730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2189227..2189789
• Replicon: chromosome
• Accession: NC_021740
• Organism: Mycobacterium tuberculosis EAI5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: M943_RS10145
• Protein ID: WP_003409778.1
• Coordinates: 2189227..2189556 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: M943_RS10150
• Protein ID: WP_023637423.1
• Coordinates: 2189553..2189789 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M943_RS10125	2185610..2186680	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
M943_RS10130	2186677..2187192	+	516	WP_003409718.1	flavin reductase family protein	-
M943_RS10135	2187189..2188250	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
M943_RS10140	2188247..2189017	+	771	WP_003409775.1	SDR family oxidoreductase	-
M943_RS10145	2189227..2189556	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M943_RS10150	2189553..2189789	-	237	WP_023637423.1	hypothetical protein	Antitoxin
M943_RS10155	2189926..2190516	-	591	WP_003409784.1	SEC-C domain-containing protein	-
M943_RS10160	2190571..2191935	+	1365	WP_003903691.1	HNH endonuclease	-
M943_RS10165	2192090..2192542	-	453	WP_003899095.1	lipoprotein	-
M943_RS10170	2192606..2193007	+	402	WP_003409869.1	hypothetical protein	-
M943_RS10175	2193000..2193182	-	183	WP_003409870.1	hypothetical protein	-
M943_RS10180	2193296..2193646	-	351	WP_003409871.1	hypothetical protein	-
M943_RS10185	2193657..2194559	-	903	WP_003409874.1	hypothetical protein	-
M943_RS10190	2194580..2194771	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T29730 WP_003409778.1 NC_021740:c2189556-2189227 [Mycobacterium tuberculosis EAI5]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T29730 NC_021740:c2189556-2189227 [Mycobacterium tuberculosis EAI5]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8455.36 Da
• Isoelectric Point: 4.5417

>AT29730 WP_023637423.1 NC_021740:c2189789-2189553 [Mycobacterium tuberculosis EAI5]
MNRIRLSTTVDAALLTSARDMRAGITDAALIDEALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 237 bp

>AT29730 NC_021740:c2189789-2189553 [Mycobacterium tuberculosis EAI5]
ATGAATCGCATACGGCTGAGCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGA
CGCAGCCCTCATCGATGAGGCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCT
ACGACAAGCACCCGGTCGATGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7